What is the net yield of ATP when each of the substrates is completely oxidized to CO₂ by a mammalian cell homogenate? Assume that glycolysis, the citric acid cycle, and oxidative phosphorylation are fully active. pyruvate: lactate: 2 12.5 fructose 1,6-bisphosphate: mannose: phosphoenolpyruvate: 13.5 34 32 dihydroxyacetone phosphate: 16 ATP molecules ATP molecules ATP molecules ATP molecules ATP molecules ATP molecules
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- What is the yield of ATP when each of the following substrates is completely oxidized to CO2 by a mammalian cellhomogenate? Assume that glycolysis, the citric acid cycle and oxidative phosphorylation are fully active. a) fructose-1,6-bisphosphate b) dihydroxyacetone phosphateIn which of the following metabolic conversions is ATP “generated” during glycolysis? Phosphoenolpyruvate -> pyruvate 2-Phosphoglycerate —> Phosphoenolpyruvate Glucose-6-phosphate —> Fructose-6-phosphate Fructose-1,6-bisphosphate —> Dihydroxyacetone phosphate + Glyceraldehyde-3-phosphate Fructose-6-phosphate —> Fructose-1,6-bisphosphateDetermine the ATP production of glucose catabolism by glycolysis and Krebs Cycle using the following information: 1. Glycolysis: Net 2 ATP, 2 NADH + H+2. Pyruvate --> acetyl CoA: Produces 2 NADH + H+/glucose3. Krebs Cycle --> 2 FADH2 + 2 ATP + 6 NADH + H+/glucose 2.5 ATP are produced/NADH + H+ delivered electron to the electron transport system1.5 ATP are produced/FADH2 delivered electron to the electron transport system
- In which of the following metabolic conversions is ATP “generated” during glycolysis? Glucose —> Glucose-6-phosphate 1,3-Bisphosphoglycerate —> 3-Phosphoglycerate Fructose-1,6-bisphosphate —> Dihydroxyacetone phosphate + Glyceraldehyde-3-phosphate Glucose-6-phosphate —> Fructose-6-phosphate 2-Phosphoglycerate —> 3-PhosphoglycerateGlycerol backbone of a fat molecule can be converted to glyceraldehyde-3-phosphate, an intermediate in glycolysis. Assuming that the single glyceraldehyde-3-phosphate undergoes complete oxidation to CO₂, how many ATPs are produced by oxidative phosphorylation only (i.e. exclude ATPs from substrate level phosphorylation) from a single fat molecule? O 20 O 18 O 17 O 15 08Consider a cell with ATP synthase deficiency (Assume that the deficiency is isolated and will not influence the function of other respiration components). In these cells/tissues, determine the following from the catabolism of the 2.5 moles of the disaccharide lactose (will be hydrolyzed first to yield glucose and galactose). Net ATP from glycolysis ATP from oxidative decarboxylation (if applicable) ATP formed from Krebs cycle (if applicable) Total net ATP
- Place the following molecules in the order in which they appear during aerobic respiration: Citrate G3P Glucose-6-phosphate Pyruvate Fructose-1,6-bisphosphate 1,3-bisphosphoglycerate 3-phosphoglycerate Glucose Acetyl-CoAWhat is the process that sustains the redox balance of glycolysis under aerobic conditions? transport of lactate from the cell regeneration of NAD* in the citric acid cycle formation of NADH in the reaction of glyceraldehyde 3-phosphate dehydrogenase regeneration of NAD* in fermentation processes regeneration of NAD* in the mitochondrial electron-transport chainConsider ten glucose molecules that enter a cell. How many ATP can be generated by the complete catabolism of these into CO2 and H2O? If all ten are first incorporated into glycogen, liberated from glycogen, and then fully catabolized into CO2 and H2O, does the ATP tally increase, decrease or stay the same? Consider that 1 UTP = 1 ATP. Explain. Describe the processes which produce ATP and provide a balanced equation of glucose, CO2, H2O and O2
- Assuming that the glycerol 3-phosphate shuttle is operative, a complete oxidation of three molecules of glucose (by a eukaryotic cell) into carbon dioxide and water would produce approximately ATP molecules via oxidative phosphorylation only. (Consider NADH = 2.5 ATP and FADH2 = 1.5 ATP)Lactate dehydrogenase (LDH) plays an essential role in an exercising muscle, especially when the level of excursion surpasses the ability of circulatory systems ability to supply adequate oxygen. What is the main reason LDH is so important to the muscle under these conditions? It produces one mole of ATP via substrate level phosphorylation. It resupplies NAD+ to glyceraldehyde 3-phosphate dehydrogenase. It decreases pH thus decreasing the dissociation rate of oxygen from hemoglobin It produces NADH for energy while converting pyruvate into lactate. It removes excess lactic acid from the muscle to create pyruvate. NextWhen fructose present in either liver or muscle undergoes glycolysis to form pyruvate, how many moles of ATP are consumed to activate it? 1 2 4 none b When glyceraldehyde-3-phosphate undergoes glycolysis to form pyruvate, how many moles of ATP are produced? 1 2 4 none c When fructose undergoes glycolysis to form two moles of pyruvate, how many moles of ATP are produced? 1 2 4 none d Is there a difference in the way fructose is metabolized in the liver versus in muscle tissue? yes no