(a)
To determine: Using the knowledge of fatty acid biosynthesis, given experimental observation: Addition of uniformly labeled [14C] acetyl-CoA to a soluble liver fraction yields palmitate uniformly labeled with [14C].
Introduction:
Fatty acid is the common pathway which occurs in the cytoplasm of the cell. Fatty acid synthesis, acetyl-CoA and NADPH produce fatty acids in the presence of enzyme fatty acid synthases.
(a)
Explanation of Solution
Explanation:
The 16 carbon molecules of palmate are derived from 8 acetyl groups of 8 acetyl-CoA molecules. The addition of [14C] acetyl-CoA will produce malonyl-CoA which is labeled at [C-1] and [C-2], followed by production of labeled palmitate. The acetyl-CoA is needed to load the acyl carrier protein, in the presence of acetyl-CoA carboxylase, acetyl-CoA, and CO2will produce malonyl-CoA.
(b)
To determine: Using the knowledge of fatty acid biosynthesis, given experimental observation: b. Addition of a trace of uniformly labeled [14C] acetyl-CoA in the presence of an excess of unlabeled
Introduction:
Fatty acid is the common pathway which occurs in the cytoplasm of the cell. Fatty acid synthesis, acetyl-CoA and NADPH produce fatty acids in the presence of enzyme fatty acid synthases.
(b)
Explanation of Solution
Explanation:
The
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