Concept explainers
To evaluate: The number of copies of a 120-kd protein required in a mammalian cell and a bacterial cell in order for it to be visible as a band on an SDS gel.
Explanation of Solution
Calculation:
Given,
Concentration of protein in cells = 200mg/ mL
Mammalian cell volume = 1000 µm3
Bacterial cell volume = 1 µm3
It is assumed that 100 µg of cell extract onto a gel and 10 ng can be detected in a single band by silver staining the gel. Thus, the calculation would be in two parts- first calculate the number of cell-equivalents that can be loaded onto gel, and then the copies of a protein that can be detected in the band.
Mammalian cell equivalents
Bacterial cell equivalents
Protein (120kD) copies in a 10 ng band
Thus to load 5×105 mammalian cells per gel and to detect 5×1010 protein molecules in a band, there must be 105 copies of protein per cell
Want to see more full solutions like this?
Chapter 8 Solutions
Molecular Biology of the Cell (Sixth Edition)
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education