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A: Introduction: Polymers are macromolecules that range from biopolymers (produced from natural sources…
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A: Colloids are formed when one substance is dispersed through another but does not combine to form a…
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- 1.Why is wavelenth the main limiting factor on limit of resoltuin in light microscopy? 2.Assuming that all other variables remain constant, explain why light of shorter wavelengths will produce a clearer image that light of longer wavelenths. 3. Why aren't the magnification of both ocular lenses of a binocular microscope used to calculate total magnification?1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?1. Please explain the characteristic differences between Bright Field Microscope and Inverted microscope.
- 1. What is an electron microscope? Mention to examples. 2. What are the main advantages and features of the electron microscope? 3. What are the main limitations or disadvantages of the electron microscope? 4. What type of specimen would you be curious to observe using an electron microscope?L of Microscopes: 1. If you have a standard ocular lens of 10x what is the true magnification that you are looking at if you are using the 100x objective lens. What is the rule for focusing if you switch to the 40x lens? 2. Calculate the sizes of the following cells. Not all cells are viewed at 40x so use the following table to calculate the size of your cells. 4x 1 reticular unit = .025 mm 10x 1 reticular unit = .01 mm 40x 1 reticular unit-.0025 mm 4 points A) Cell 1 on 10x=8 reticular units B) Cell 2 on 40 x = 28 reticular units mm mm Which one is actually larger? Which one is probably the plant cell? Why?7. What determines the resolution limit of a microscope? Is the purpose of the fluorescence microscope to increase the magnification beyond that of a typical light microscope? Why or why not? d in lah.
- Describe 3 simple ways of preparing slides for light microscopy microscope1. In microscopy, what could be the possible reason why we cannot completely resolve the specimen under the Oil Immersion Objective (OIO) when, in fact, it was taught that the OIO has a resolving poewer? 2. When using the OIO, we can only focus on one group or parts at a time. (that is, the other parts are blurred) Whys is it so?7. Why should the microscope be calibrated for each objective and prior to each use?8. When carrying the microscope, why should one hand hold the bottom of the base?
- 1. Is a microscope an absolute necessity for studying microorganisms? Why or why not? 2. What do you think are the limitations of a brightfield microscope? Why is a brightfield microscope the most common type of microscope in routine clinical laboratories? 3. A slide in which a stained specimen is placed on the microscope stage. After careful and correct manipulation of the adjustment knobs, the specimen is still not in focus. What do you think is the reason? What corrective actions can be done? (provide computation) 1. What is the formula to determine the actual size of an organism? 2. The actual size of an Escherichia coli is 1.5um. What would be its image size if the magnification used is 1000x? 3. What magnification is used if an Bacillus sp. have a microscopic size of 3.5um and an image size of 15mm? NOTE: please try to answer all of the question, i promised to give you a good ratingg1. Differentiate the compound light microscope from the dissecting microscope in terms of: *image produced *magnification *type of specimen examined 2. Write down the principles of the following: *electron microscopy *scanning tunneling microscopy *atomic force microscopydiscuss the microscopy technique that gives the best constrast image of non biological samples such as micro and nano particles?
