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1. What are the effects CCl 4 solvents when mixed with lipids?
2. Explain enzyme hydrolysis.
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- 3. What are the effects of the following solvents when mixed with lipids? What type of hydrolysis is present when mixed withthose solvents?a. waterb. CCl 4c. Etherd. CHCl 4. Explain enzyme hydrolysis.3. What are the effects of the following solvents when mixed with lipids? What type of hydrolysis is present when mixed withthose solvents? Explaina. waterb. CCl 4c. Etherd. CHClII. Lipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant - chloroform: methanol: water (6:3:1) 2nd eluant - methanol: chloroform (9:1)3rd eluant – petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Based on the results above, _________________ 1. _________________ 2. _________________ 3. _________________ 4. _________________ 5. answer the following questions: What visible positive result for test reagent no. 5?Give the name of chemical characterization test for test reagent no. 3?What class of lipids will give a visible positive result on the test mentioned in question no. 2?Give the chemical name of the product formed after giving a positive result for test reagent no. 1?Test reagent no. 1 is used to test for what organic compound?
- 1. Why should the potassium salts of fatty acids yield soft soaps?1. Provide an example of a liquid that has a pH less than 7, one that is neutral and one that is greater than 7. 2. Yeast incubated in NaCl only or Sugar solution?, at room temperature or on ice?Which of the following is MOST soluble in polar solvents? A H₂C ОН В с D ОВ O О О O A H3C. HO HC CH3 CH3 ОН COOH CH3 CH3 CH3
- 1. What is this lipid structure, give its role/ function, and enumerate its hydrolysis products or components. 2. Explain which hydrolyzed component will be positive in the Acrolein test? Ninhydrin test?What is the purpose of: A. The concentrated salt solution? B. adding isopropyl alcohol to the solution? Why does it need to be in low temperature?Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
- 4. A stock solution has a concentration of 10M. 50 mL of this stock solution is used to make 1L of a dilute solution. What is the concentration of the dilute solution? BLOC DE GRAPHISME GR 50 5. How many grams of KCl are there in 10 mL of 20% solution.D. * 3. Describe what it means that water has A. High cohesiveness, B. high adhesiveness, C. a neutral pH (using ion concentration to explain), and D. has a low viscosity.a. Is there a microbe that could grow on a medium that contains onlythe following compounds dissolved in water: CaCO3, MgNO3,FeCl2, ZnSO4, and glucose? Defend your answer.b. A useful mnemonic device for keeping track of major elementsrequired by most organisms is: C HOPKINS Ca Fe, Na ClMgood. Read aloud, it becomes “See Hopkin’s Cafe, NaCl (salt)M good!” What does each letter stand for?