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- What is the role of imidazole in Ni-Affinity Chromatography?How does HPLC differ from ionexchange chromatography?For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm Rs when H = 3 cm > Rs when H = 5 cm Rs when H = 3 cm < Rs when H = 5 cm The two quantities cannot be compared due to insufficient information.
- What does an A280 of 0.000 indicate? What does this reading indicate in the context of your column chromatography? Are there still proteins adhering to the column? Do you know anything about the proteins which are still adhering to the column?In Experiment 7, Isolation of Citrus Oils, the compounds isolated were the monoterpene compounds alpha-pinene, beta-pinene, limonene, myrcene and gamma-terpinene. The citrus oils were analyzed using gas chromatography, which separates compounds based on polarity and boiling point. Column chromatography and thin layer chromatography work on similar separation principles as gas chromatography, but rely only on polarity of compounds and solvents for separation. Explain why neither column chromatography nor thin layer chromatography would be appropriate separation methods for the terpenes isolated in Experiment 7. Answer:A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
- Briefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatographyWhat is the difference between normal and reverse phase chromatography?Using the formula V₁D₁ = V₂D2, what volume of a 1/100 (10-2) dilution would you need to produce 100 mL of a 1/10000 (10-4) dilution? (Note: provide your answer in decimal format to three decimal places.)
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?The order is for Ceftin 0.25 gPO, bid. On hand you have Ceftin 125mg tablets. How many tablets should you give?Explain why each of the following measurements is or is not the result of a Bernoulli trial: (a) The number of surgical procedures performed in a hospital in a week(b) A hospital patient’s temperature in degrees Celsius(c) A hospital patient’s vital signs recorded as normal or not normal