Describe the propagation stage of peroxidation reaction by showing peroxidation of oleic acid. Peroxidation of arachidonic acid is 200 times faster than oleic acid. Briefly explain that observation.
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- Explain the role of L-tartrate in the determination of acid phosphatase . Differentiate thymolphthalein monophosphate method from alpha naphthyl phosphate method of prostatic acid phosphate determination. Why is acid phosphatase determination useful in establishing recent sexual intrusion as in the case of alleged rape? EXPLAIN 3-5 SENTENCESPlease ASAP. Thank you. Explain these results in short answer form. Why was the slope zero for blank solution? What was the optimal concentration for peroxidase activity? Do these values make sense?Describe the two phases of the the HMP shunt. Enumerate the reactions in each phase and the corresponding enzyme of each reation. Explain how the HMP shunt is regulated?
- Table 1 - Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract Absorbance 0 mins after 30mins (2nd reading) (mL) 1st reading 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 0.00 0.060 0.033 0-05-2 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Time for reading: 3:21 -0.11 Absorbance: Time for reading: 3.36 Q5) Tube la serves as a control, but why is this control needed? Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: . 166 ness Catechol Benzoquinone Subtract 1st from 2nd reading -0.01 0-137 0.11.19 0.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…Describe each step of the metabolic pathway shown in the image below. Linoleic Acid Metabolism 18:2n-6, HO. Linoleic Acid 18:3n-6 он Gamma Linolenic Acid 20:3n-6 он Dihomo Gamma Linolenic Acid 20:4n-6 HO, Arachidonic Acid 22:4n-6 но, Docosatetraenoic AcidName the components of the hexose-monophosphate pathway that detoxify peroxide and the process that accomplishes detoxification.
- Explain why the hexose monophosphate shunt is so important.What is the approximate rate of change of A340 measured? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 30 ml of the ADH solution was tested in the same way (i.e. half the amount of protein) ? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 60 ml of a 0.5 μM ADH solution was tested in the same way? i.e. ΔA340 / min = __________ As well as writing your answers, explain your reasoning.Define the following terms:a. superoxide dismutaseb. peroxiredoxinc. thioredoxind. b-carotenee. ascorbate