Parts A-C in the table show the genotypes for two partial diploid strains of the lac operon. Gene names have been simplified (i.e., LacZ is shown as Z). Alleles are shown using superscript notations. There are two copies of the operon, one line is the copy in the genome and the other line is in a plasmid. In the blanks, indicate the effect of the partial diploid strains' genotype on the expression of the structure gene specified in the header of the table. Choose "functional" if you expect that any amount of normal enzyme will be made, "nonfunctional" if you expect that transcription occurs but only the mutated enzyme is made, or "no transcription" if the structural gene is not transcribed. Assume glucose is absent. Genotype (A) (B) (C) I- P+ O+ Z+Y- I+ P+ O+ Z- Y+ I- P+ OC Z+Y- I+ P- O+ Z-Y+ IS P+O+ Z+Y- I+ P+ OC Z- Y+ Lacy Presence of Lactose? 1. [Select] 3. [Select] 5. [Select] > > < Absence of Lactose? 2. [Select] 4. [Select] 6. [Select] < > >

Parts A-C in the table show the genotypes for two partial diploid strains of the lac operon. Gene names have been simplified (i.e., LacZ is shown as Z). Alleles are shown using superscript notations. There are two copies of the operon, one line is the copy in the genome and the other line is in a plasmid. In the blanks, indicate the effect of the partial diploid strains' genotype on the expression of the structure gene specified in the header of the table. Choose "functional" if you expect that any amount of normal enzyme will be made, "nonfunctional" if you expect that transcription occurs but only the mutated enzyme is made, or "no transcription" if the structural gene is not transcribed. Assume glucose is absent. Genotype (A) (B) (C) I- P+ O+ Z+Y- I+ P+ O+ Z- Y+ I- P+ OC Z+Y- I+ P- O+ Z-Y+ IS P+O+ Z+Y- I+ P+ OC Z- Y+ Lacy Presence of Lactose? 1. [Select] 3. [Select] 5. [Select] > > < Absence of Lactose? 2. [Select] 4. [Select] 6. [Select] < > >

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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The genotypes of five merozygote bacteria are shown below. In each set, the genotype of the lac operon on the chromosome is on the left side of the slash (/) and the genotype of the lac operon on the F' plasmid is on the right side of the slash. Which of the merozygote bacteria listed below would be able to make high quantities of B-galactosidase in the absence of lactose? Mark all the answers that are correct. OIP*o*z* y*A*/F'I P oʻz*Y*A*. OI P o°z-Y*A*/F'IS P O*z*Y*A* nd of B * P*o*zY A /F'I P*o z*Y*A* erase e D I' P*o*z YA /F'I P*O z*Y* A* strand a NA poly should be 1S P*0°ZYA/FI P O*z*Y*A*
A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the lowest beta-galactosidase enzyme activity, when grown in the lactose medium. OF POZY Ort Ptot Z¹ Yt Ort p²o+z¹Y+ Orpt ot zty
The streptolysin S toxin made by S. pyogenes is encoded by a 9-gene operon, sagABCDEFGHI. Thinking about what a 3-line diagram would look like for this operon, answer the following questions. Write numeric answers only. For example, if your answer is 6 promoters, write only 6. 1) How many promoters control the expression of these genes? 2) How many locations does RNA Polymerase bind to get full expression of these genes? 3) How many ribosome binding sites are needed for full protein expression? 4) How many start codons will be needed for full protein expression? 5) How many mRNA strands will be produced with full operon expression? 6) How many proteins will be produced with full protein expression? 1
The genotypes of five merozygote bacteria are shown below. In each set, the genotype of the lac operon on the chromosome is on the left side of the slash () and the genotype of the lac operon on the F' plasmid is on the right side of the slash. Which of the merozygote bacteria listed below would be able to make high quantities of B-galactosidase in the absence of lactose? Mark all the answers that are correct. O i p*o°z¯ y*A*/F'I$ p*oʻz*y*A* I* p* o*Z¯YA*/F'T P*O*z*y* A* I* P* o*z*Y* A* /F'T P*O*z*y* A* O 1* p*o*z° YA*/F'T P*o°z*y*A* 1S p*0°2¯Y¯AZET P*O*Z*Y*A*
The following table lists 4 bacterial strains that are partial diploids for lac operon genes. Given the activity of beta-galactosidase measured for each strain in the absence (-lac) or presence (+lac) of lactose, complete the table by choosing the appropriate symbol (+, -, C, S) to indicate the allele of the gene or site missing from the table (blue numbers). strain A BC 5 C D 7 chromosome I O 1 2 4 1 [Select] 9 3 [Select] [Select] [Select] 9 [Select] + + Z + + 6 + I +5 + 10 plasmid O 3 + 7 C Z + 8 8 2 [Select] 4 [Select] 6 [Select] B-gal act. -lac +lac 0.002 0.003 0.002 0.058 0.063 0.121 0.059 0.062 Select] 1 ✔ [ Select] + is C S
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E,coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/). The possible answers indicate with a ʺ+ʺ or a ʺ-ʺ whether β-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in the presence of lactose. (Assume that glucose is not present in the medium.)Genotype F: I+ Oc Z-/ Fʹ I- O+ Z+ KEY:I+ = wild-type repressorI- = mutant repressor (unable to bind to the operator)Is = mutant repressor (insensitive to lactose)O+ = wild-type operatorOc = constitutive operator (insensitive to repressor)
The map of the lac operon is shown below. Consider the following examples that include both haploids and partial diploids and explain in each scenario whether the repressor can bind and regulate expression and whether or not the lac operon is expressed. For partial diploids the plasmid is indicated by the F’. I+ O+ Z+ Y+ / F’ I+ O+ Z+ Y+ I- O+ Z+ Y+ / F’ I+ O+ Z+ Y+ I- O+ Z+ Y+ I+ Oc Z+ Y+
The symbols A, B, C, D, E represent all the components of the lac operon, excluding LacA. The symbols are not in the order of the lac operon. Minus and plus signs represent activity of lacZ or LacY in the absence or presence of IPTG. LacI+ or LacI-, and LacO+ or LacOC alleles were used. Which of the symbols represents each of the lac operon components?
A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the highest beta-galactosidase enzyme activity, when grown in the lactose medium. CAP It P+ O`Z+ CAP+ I P+ O+ Zt CAP+ It P- O+ Z+ CAP+ It P+ 0+ Z
A strain of E. coli has the genotypes shown below at the lac operon, where I = regulator gene, P = promoter, O = operator, Z = βgalactosidase gene, and Y = permease gene. The superscript+ indicates a wild-type allele,c indicates a constitutive mutation, and− indicates a defective mutation. For each genotype, indicate whether the enzyme will be synthesized or not synthesized when lactose is present or absent by placing a + for synthesis occurring and a − for synthesis not occurring in the appropriate blank. Explain your reasoning for each answer.
The following table lists 4 bacterial strains that are partial diploids for lac operon genes. Given the activity of beta-galactosidase measured for each strain in the absence (-lac) or presence (+lac) of lactose, complete the table by choosing the appropriate symbol (+, -, C, S) to indicate the allele of the gene or site missing from the table (blue numbers). + = wildtype, - = null mutation, c = constitutive, s =super repressor chromosome plasmid B-gal act. strain 10 Z A 1 C B 3 4 + C + 6 D 9 + 1 [Select] 3 [Select] 5 [Select] 7 [ Select] 9 [Select] | 0 2 + + 5 + 7 10 Z + -lac +lac 0.062 0.058 0.003 0.004 0.062 0.117 0.003 0.060 + 8 + 2 [Select] 4 [Select] 6 [Select] 8 [Select] 10 [Select]
For the given genotypes (associated with the lac operon in E. coli), indicate with a "+" or "-" whether functional B - galactosidase would be expected to be produced in the indicated conditions, assuming low glucose levels. B - galactosidase production Genotype No lactose With lactose a) I- O+ Z+ / F' I+ O+ Z+ b) IS O+ Z+/ F' I+ O+ Z+