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- Match the following term with its description or definition. Immunoglobulin domain Amino acids that are separated in the protein chain but come together in the folded protein Antigen that carries several epitopes of the same or different specificity Heavy chain classes Soluble proteins that carry only one copy of the epitope ✓ Choose... IgG, IgM, IgD, IgA, IgE Multivalent antigen Discontinuous epitope Monovalent antigen Repeated barrel-shaped structure Choose...You are solving for an unknown blood type. Drops of blood have been mixed with solutions containing the antibodies Anti- A, Anti-B and Anti-Rh. The test results are are pictured below. Using your knowledge of antibody- antigen interactions and the principle of agglutination, identify the blood type and answer the associated questionsYou are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodamine
- In antigen-antibody ratio,these two zonal phenomenon/reaction in precipitation can result into false negative serologic results. Justify your answer.You are seeing a 4-year old girl who has a 6-week history of recurrent left knee pain and a limp. On examination, the joint is red, warm and tender but she is able to weight bear with a limp. Of the following, this girl is MOST LIKELY to test positive for:a. Anti-nuclear antibodyb. Rheumatoid factorc. Anti-double stranded DNA antibodyd. Anti-streptolysin O antibodyasap Which of the following would not be consistent with Infectious Mononucleosis (IM): a Absolute Lymphocyte count of <4.0 x 109/L b Relative Lymphocyte count usually >50% c Greater than 20% reactive lymphocytes counted on manual differential d Positive heterophile antibody test.
- Which of the following statements is the most accurate concerning lymphocytes? a. T cells and B cells both express TCRs. These are the product of approximately 100 genes. Each lymphocyte expresses only one of the ~100 receptors coded for by these genes, and in the absence of clonal selection the liklihood that two cells express the same receptor is only moderate b. T cells and B cells express TCRs and BCRs respectively. These are the product of genetic recombination and can comprise one of millions of combinations. Each lymphocyte can express 1000s of different receptor variants, maximizing the likelihood that a single adaptive immune cell will recognize pathogen-associated antigens. c. T cells and B cells express TCRs and BCRs respectively. These are the product of genetic recombination and can comprise one of millions of combinations. Each lymphocyte expresses only one of these receptors, and in the absence of clonal selection the liklihood that two cells express the same…2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?Upon antigen exposure, antigen binding sites;. is the first antibody produced with is the second antibody produced with antigen binding sites, and the is the last antibody produced, which is most specific and has antigen binding sites O IgM; 10; IgA; 4; IgG; 2 O IgM; 10; IgD; 4; IgA; 2 IgM: 2; IgA: 2; IgG: 2 O IgG; 2; IgE; 4: IgM: 10
- Which of the following statements pertain to this cell. This cell is a precursor to a cell which: 1. is an antigen-presenting cell 2. demonstrates MHC I molecules 3. demonstrates MHC II molecules 4. demonstrates CD4 molecules 5. is capable of synthesizing and releasing perforin and granzymes Choose from the following: (A) 1, 2, and 3 (B) 2 and 3 only (C) 3 and 4 only (D) 1 and 3 (E) 1, 4, and 5What is likely to be the problem if after you add substrate, all the wells begin to change to a deep color, including your controls? You may choose more than one the washing was not sufficient the antibodies used to coat the wells are not binding the antigen O you may not have blocked the wells appropriately O the antibodies used to coat the wells are not specific to the antigen and bind many serum proteins1.A given B cell expresses only maternally or paternally derived heavy chains but never both. This observation is the result of A. antibodydiversity.B. isotypeswitching.C. allelicexclusion.D. affinitymaturation.E. randomVJgenerearrangement. 2.Which of the following antibody classes or isotypes facilitate the sequential binding of the C1, C4, C2, and C3 components of the complement system? A. IgAandIgD B. IgAandIgE C. IgDandIgM D. IgE and IgG E. IgMandIgG