Which spectrophotometer mode should you use to calibrate the instrument and which mode should you use to read your samples? Transmittance for both Absorbance for both Absorbance to calibrate and transmittance to read samples Transmittance to calibrate and absorbance to read samples Flip a coin to pick a mode each time.
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Q: Question attached
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Transmittance to calibrate and absorbance to read samples
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Flip a coin to pick a mode each time.
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- Which of the following statements are true about the use of a blank in spectrophotometry? Select all that apply. Select 2 correct answer(s) A blank is needed because the spectrophotometer also measures the absorbance of the cuvette and water. A blank must be measured prior to measuring your sample. None of the answer choices listed are correct. A blank must be measured after measuring your sample. A blank is only needed when the concentration of your sample is known.Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700Which of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.
- Assume your field of view under a 3X objective is 6mm across. What is the field of view in mm under a 20X objective? Show clear work with units for credit. Field of view 20X objective? ___mm What is the field of view of this 20X objective in micrometers? If you observe a specimen under the 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?If you observe a specimen under this 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?
- What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?Here are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.During studying a thick heavily stained specimen under the microscope, it is better to light intensity adjustment has nothing to do with the clarity of the specimen. increase the light intensity passing through the specimen decrease light intensity passing through the specimen none of the options is correct
- Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourceActivity: Calculate the magnification for the following. Actual Specimen Size Image Size Magnification 0.5 μm 2 cm 200 μη 1 cm 10 cm 25 x 4 cm 50 x 4 mm 3 xGiven: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.