Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 12.2, Problem 1COMQ
With regard to a promoter, a transcriptional start site is
a. located at the
sequence and is recognized by
factor.
b. located at the
sequence and is where the first base is used as a template for transcription.
c. located at the
site and is recognized by σ factor.
d. located at the
e. site and is where the first base is used as a template for transcription.
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Within a promoter, a transcriptional start site isa. located at the –35 sequence and is recognized by σ factor.b. located at the –35 sequence and is where the first base is used as atemplate for RNA transcription.c. located at the +1 site and is recognized by σ factor.d. located at the +1 site and is where the first base is used as a templatefor RNA transcription.
a. What is grey donut shaped object supposed to represent?
b. Label with the word "promoter" where one might find the promoter region in this image.
c. In the magnified insert, label the template strand that is being transcribed with the word
"template".
d. In the magnified insert label the 5' and 3' ends of the transcript.
e. What entity functions to break the complementary base pairing of a gene at its
transcription start site?
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Eukaryotic transcription
A. is independent of the presence of upstream consensus sequences.
B. may involve a promoter located within the region transcribed rather than upstream.
C. requires a separate promoter region for each of the three ribosomal RNAs transcribed.
D. requires that the entire gene be in the nucleosome form of chromatin.
E. is affected by enhancer sequences only if they are adjacent co the promoter .
Chapter 12 Solutions
Genetics: Analysis and Principles
Ch. 12.1 - 1. Which of the following base sequences is used...Ch. 12.1 - Prob. 2COMQCh. 12.2 - With regard to a promoter, a transcriptional start...Ch. 12.2 - Prob. 2COMQCh. 12.2 - 3. Sigma factor is needed during which stage(s) of...Ch. 12.2 - A uracil-rich sequence occurs at the end of the...Ch. 12.3 - Which RNA polymerase in eukaryotes is responsible...Ch. 12.3 - Prob. 2COMQCh. 12.3 - Prob. 3COMQCh. 12.3 - Prob. 4COMQ
Ch. 12.4 - Which of the following are examples of RNA...Ch. 12.4 - A ribozyme is a. a complex between RNA and a...Ch. 12.4 - Prob. 3COMQCh. 12.4 - Prob. 4COMQCh. 12.5 - 1. Which of the following is not a key difference...Ch. 12 - Prob. 1CONQCh. 12 - Prob. 2CONQCh. 12 - Prob. 3CONQCh. 12 - Prob. 4CONQCh. 12 - 5. Mutations in bacterial promoters may increase...Ch. 12 - Prob. 6CONQCh. 12 - 7. In Chapter 9, we considered the dimensions of...Ch. 12 - 8. A mutation within a gene sequence changes the...Ch. 12 - Prob. 9CONQCh. 12 - At the molecular level, describe how factor...Ch. 12 - Prob. 11CONQCh. 12 - What is the complementarity rule that governs the...Ch. 12 - 13. Describe the movement of the open complex...Ch. 12 - 14. Describe what happens to the chemical bonding...Ch. 12 - Prob. 15CONQCh. 12 - Prob. 16CONQCh. 12 - Prob. 17CONQCh. 12 - Mutations that occur at the end of a gene may...Ch. 12 - If the following RNA polymerases were missing from...Ch. 12 - 20. What sequence elements are found within the...Ch. 12 - 21. For each of the following transcription...Ch. 12 - 22. Describe the allosteric and torpedo models for...Ch. 12 - Which eukaryotic transcription factor(s) shown in...Ch. 12 - 24. The initiation phase of eukaryotic...Ch. 12 - A eukaryotic protein-encoding gene contains two...Ch. 12 - 26. Describe the processing events that occur...Ch. 12 - Prob. 27CONQCh. 12 - Prob. 28CONQCh. 12 - Prob. 29CONQCh. 12 - Prob. 30CONQCh. 12 - 31. In eukaryotes, what types of modifications...Ch. 12 - Prob. 32CONQCh. 12 - Prob. 33CONQCh. 12 - 34. Figure 12.21 shows the products of alternative...Ch. 12 - 35. The processing of ribosomal RNA in eukaryotes...Ch. 12 - Prob. 36CONQCh. 12 - Prob. 37CONQCh. 12 - After the intron (which is in a lariat...Ch. 12 - Prob. 1EQCh. 12 - 2. Chapter 21 describes a technique known as...Ch. 12 - Prob. 3EQCh. 12 - As described in Chapter 21 and in experimental...Ch. 12 - Prob. 5EQCh. 12 - Prob. 6EQCh. 12 - 1. Based on your knowledge of introns and pre-mRNA...Ch. 12 - Discuss the types of RNA transcripts and the...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Regulatory proteins in Prokaryotes shut off transcription by binding to a site immediately in front of the promoter and often even overlapping the promotor. This site is referred to as the A. suppressor site. B. operator site. C. co-activator site. D. regulatory site.arrow_forwardA high rate of transcription initiation from promoters in bacteria can be achieved by which of the following? a. the presence of perfect consensus sequences at the -35 and -10 positions of the promoter. b. the binding of an activator protein close to the promoter. c. the presence of an UP element immediately upstream of the promoter. d. a and c, but not b e. a, b, and carrow_forwardWhich of the following is false regarding the role of mediator complex in eukaryotic transcription initiation? a. Mediator complex interacts with RNA pol II b. Mediator binds to upstream enhancer sequences c. Mediator interacts with general transcription factors d. Mediator interacts with transcriptional activators e. All of the above are true about the mediator complexarrow_forward
- Transcription of eukaryotic genes requires the presence of a promoter and usually the presence of enhancers. An enhancer: A. is a consensus sequence in DNA located where RNA polymerase first binds. B. may be located in various places in different genes. C. may be on either strand of DNA in the region of the gene. D. functions by binding RNA polymerase. E. stimulates transcription in both prokaryotes and eukaryotes.arrow_forwardWhich of the following is not true regarding gene regulation that involves DNA bending? a. The precise distance between the regulatory sequence and the promoter is important. b. Effect can be to repress transcription c. Effect can be to activation transcription d. Regulated genes can be thousands of base pairs away from the regulatory sitesarrow_forwardWhich of the following is generally true about eukaryotic gene regulatory regions? a. All regulatory regions lie within 200 base pairs of the promoter. b. Promoters are typically under control of a single regulatory sequence. c. Promoters are usually blocked through the action of repressors d. Enhancer sequences can be located thousands of base pairs upstream or downstream from the promoter.arrow_forward
- Which of the following activities DOES NOT happen during the initiation stage of transcription? A. Use of RNA polymerase. B. Detection of TATA-box C. Attachment of transcription factors D. Addition of G' Cap and Poly-A tail A section of DNA has the following sequence of nitrogenous bases: CGATTACAG. Which of the following sequence would be produced as a result of transcription? A. CGTUUTCTG B. GCTAATGTC C. CGAUUACAG D. GUAAUGUCarrow_forwardConsider a mutation in -10 BOX promoter consensus sequence (TATAAT) in the prokaryote recA gene where the first T base mutated to G, what outcome(s) are likely? a. transcription levels will increase as the GC content would increase b. this mutation is silent, so no change in transcription level c. transcription levels would decrease because the promoter would be weaker d. transcription levels do not depend on the promoter sequence for the gal gene e. nothing would happen because the promoter would not change the mRNA sequencearrow_forwarda. The promoter is to the right so transcription goes right to left.b. This sequence 5’ AGCTACGGGGTG 3’ is a part of the template strand from the MIDDLE of gene. Write it in the middle of one of your lines. Use pencil in case of errors. c. Write the sequence of the coding strand of DNA on the other line. d. Indicate the start of transcription with an arrow like this and write “promoter” where it must be located. e. Draw a thicker (or different colored) line to indicate a strand of newly synthesized mRNA. Label 5’ and 3’ ends. By the template sequence on your diagram, write the sequence of mRNA that is produced from this template. f. In what ways are the sequences of the coding strand and the mRNA similar? g. In what ways are the coding strand and the mRNA different?arrow_forward
- After treating cells in tissue culture with an HDAC inhibitor, which of the following is the most likely outcome? A. Repression of transcription B. Promoter methylation C. Enhanced splicing D. Activation of transcription E. Depends on the cell typearrow_forwardThe promoter consensus sequence at the -10 BOX in the bio gene in E. coli was TAGACT; however, a transversion occurred which changed the G nucleotide to T, what outcome(s) are likely? a. transcription levels would decrease because the promoter would be weaker b. nothing would happen because the promoter would not change the mRNA sequence c. the mutation would block RNA polymerase from binding to the DNA d. transcription levels would increase because the promoter would be stronger e. the bio gene does not require a functional promoterarrow_forwardChoose the item in column 2 that best matches each item in column 1. A. Provides information for production of protein B. Binds to promoter C. Promoter has a box A and box B consensus sequences D. Autocatalytic RNA molecules E. Associated with transcription termination F. Polymerization of ribonucleotides to form RNA molecules G. 7-methyl guanosine H. MRNA prior to processing select - 1. Core RNA polymerase select 2. rho (p) factor select 3. hnRNA select - 4. RNA select -5. RNA polymerase holoenzyme select 6. Ribozymes select - 7. 5' MRNA cap select 8. MRNAarrow_forward
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