Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 28.2, Problem 2TYK
Summary Introduction
Introduction:
Rb genes are found in animals and plants which repress the cell proliferation. This gene is in activated form and controls the onset of cellular differentiation in number of cell types. In animals the Rb gene is part of conserved pathway controlling the activation of cell division in animals.
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You conduct an experiment to study the expression of the S protein through the
tissues of your favorite plant (Arabidopsis thaliana). The morning of the experiment
you inject in the leaves a messenger RNA that codes for the synthesis of a single
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and a red fluorescent protein tag (RFP). You perform two cross sections of the same
root, one section at the start of the experiment (time = Oh; corresponding to the time
of injection) and one section in the afternoon (time = 8h). Through fluorescent
microscopy you observe a change in the coloration inside the cells of the root's
central tissues (as indicated by the arrows): from no coloration (time = Oh) to red
(time = 8h).
• The cells of the central tissues in the roots do not have nuclei or ribosomes.
How can you explain this change of coloration? Please provide a cellular
feature that can lead to this.
• What is one advantage of using an RFP-tag in an mRNA?…
You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a
mutagenesis screen to identify mutants in the photoperiod pathway. Given what you know about
photoperiodism in Arabidopsis, what phenotype are you looking for and under what photoperiodic
conditions would you perform the experiment?
delayed flowering in long days
delayed flowering in short days
same flowering in short days
early flowering in short days
same flowering in long days
early flowering in long days
In roses, the synthesis of red pigment is produced by two steps in a pathway.
gene O
magenta intermediate -
gene P
colorless intermediate-
red pigment
What would the phenotype be of a plant homozygous for a null mutation of gene P?
What would the phenotype be of a plant homozygous for a null mutation of gene Q?
What would the phenotype be of a plant homozygous for null mutations of genes P and Q?
magenta
red
Match a genotype to each strain.
colorless
Strain
P locus Q locus
homozygous null mutation of gene P
homozygous null mutation of gene Q
homozygous null mutations of genes P and Q
Answer Bank
plp
PIP
What F2 ratio is expected from crossing a plant that is homozygous for a null mutation of gene P with a plant that is
homozygous for a null mutation of gene Q? Assume independent assortment.
9 colorless : 4 magenta : 3 red
9 red : 4 colorless : 3 magenta
O 9 red : 4 magenta : 3 colorless
Chapter 28 Solutions
Principles of Biology
Ch. 28.1 - Refer back to Figure 25.4 to see the life cycle...Ch. 28.1 - Prob. 1TYKCh. 28.1 - Prob. 2TYKCh. 28.2 - Prob. 1CCCh. 28.2 - Prob. 2CCCh. 28.2 - Prob. 1TYKCh. 28.2 - Prob. 2TYKCh. 28.3 - Prob. 1CCCh. 28.3 - Prob. 2CCCh. 28.3 - Prob. 3CC
Ch. 28.3 - Prob. 1TYKCh. 28.3 - Prob. 2TYKCh. 28.4 - Prob. 1TYKCh. 28.4 - Prob. 2TYKCh. 28.4 - Prob. 1CCCh. 28 - Where would you look to find the gametophyte...Ch. 28 - Prob. 2TYCh. 28 - Prob. 3TYCh. 28 - Prob. 4TYCh. 28 - Prob. 5TYCh. 28 - Prob. 6TYCh. 28 - Prob. 7TYCh. 28 - Prob. 8TYCh. 28 - Prob. 1CCQCh. 28 - Prob. 2CCQCh. 28 - Prob. 3CCQCh. 28 - Prob. 1CBQCh. 28 - Prob. 2CBQ
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