Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 9, Problem 1CA
Endospores appear as colorless areas in a simple stain and in a
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What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.
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Endospores can be seen as refractile structures in unstained cells and as colorless areas in Gram-stained cells. Why is it necessary to do an endospore stain to verify the presence of endospores?
Chapter 9 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 9 - What are the Gram reactions of Clostridium and...Ch. 9 - How might a capsule contribute to pathogenicity?...Ch. 9 - Prob. 3QCh. 9 - The flagella stain uses crystal violet, as does...Ch. 9 - How would an endospore stain of Mycobacterium...Ch. 9 - What type of culture medium would increase the...Ch. 9 - Prob. 3CTCh. 9 - Prob. 4CTCh. 9 - Endospores appear as colorless areas in a simple...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is the difference between simple and differential stains? Give an example of a differential stain.arrow_forwardAbout the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?arrow_forwardwhy is it necessary to employ a spore stain rather than rely on diagnosis of sporing from examination of gram stainarrow_forward
- What is the purpose of the alcohol (OH) in the gram staining process? Explain.arrow_forward1) In Gram staining, to what cell structure do the dyes bind? 2) Would it be useful to perform a Gram stain on a mixed culture? Why? 3) In capsule staining, why does the capsules did NOT take in any dye? 4) In endospore staining, what is the purpose of using the steam?arrow_forwardwhat are the positive and negative results of the endospore stain procedure?arrow_forward
- What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.arrow_forwardIs it possible to perform double or triple staining using fluorescent dyes? Explain.arrow_forwardDescribe the bacteria observed in different staining procedures. Please refer to the photo - Direct Stain - Negative Stainarrow_forward
- Why is it necessary to perform a negative stain to visualize capsules?arrow_forwardAdditional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?arrow_forwardWhat's the difference between the gram stain of Bacillus megaterium and Bacillus Subtilis?arrow_forward
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