Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 9, Problem 4CT
Summary Introduction
To determine:
The microscopic appearance of the result of the Dorner endospore stain.
Introduction:
When the bacterial cells are in a dormant state, then they form endospores. In dormancy, cells do not undergo
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Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)
a
acid alcohol
b
crystal violet
c
carbolfuschin
d
malachite green
e
safranin
f
iodine
g
alcohol
h
methylene blue
What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.
Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
Cite the purpose of each of the following reagents in a differential staining procedure.
Primary stain
Mordant
Decolorizing Agent
Counter stain
What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day
Chapter 9 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 9 - What are the Gram reactions of Clostridium and...Ch. 9 - How might a capsule contribute to pathogenicity?...Ch. 9 - Prob. 3QCh. 9 - The flagella stain uses crystal violet, as does...Ch. 9 - How would an endospore stain of Mycobacterium...Ch. 9 - What type of culture medium would increase the...Ch. 9 - Prob. 3CTCh. 9 - Prob. 4CTCh. 9 - Endospores appear as colorless areas in a simple...
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- what are the positive and negative results of the endospore stain procedure?arrow_forwardWhich of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.arrow_forwardWhat color wouod gram negative cells appear if the decolorizing step was left out of staining procedure? Why?arrow_forward
- Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct Stain - Negative Stainarrow_forwardIs heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?arrow_forwardIn preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?arrow_forward
- What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.arrow_forwardThe bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forwardDefine the term gram staining.arrow_forward
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