19. Endorphins are polypeptides that reduce pain. What is the amino acid order for the following mRNA, which codes for a pentapeptide that is an endorphin called leu- enkephalin? 5'AUG UAC |GGU | GGA UUU | CUA | UAA3'
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- The following is a portion of a protein: met-trp-tyr-arg-gly-pro-thr-Various mutant forms of this protein have been recovered. Using the normal and mutant sequences, determine the DNA and mRNA sequences that code for this portion of the protein, and explain each of the mutations. a. met-trp- b. met-cys-ile-val-val-leu-gln- c. met-trp-tyr-arg-ser-pro-thr- d. met-trp-tyr-arg-gly-ala-val-ile-ser-pro-thr-6. How many amino acids will the mRNA sequence "AUG GÁC CUG UCG UGA" produce? (LS1-1) * Second mRNA base G UUU Phe UUC UCU UAU UGU Tyr UAC Cys UCC Ser UCA UGC C Phe Gly (G) Leu UAA Stop UGA Stop A Glu (F) (L) UUA Leu UUG Ser Asp (E) (D) (S) Tyr (Y) UCG UAG Stop UGG Trp GUC Ala CUU (A) A CCU CAU CGU His CUC Leu CUA CC Pro ССА CAC CGC Arg CGA Cys (C) Val CAA Gln CAG G U (V) G Trp (W) CUG CCG CGG Arg (R) G U A C A C Leu AUU ACU AAU Asn AGU Ser AGC (L) Ser (S) C AUC Ile ACC AAC Thr Lys (K) UG A AUA Pro AGA Arg AGG ACA AAA Lys AAG Asn (N) (P) AUG Met or start ACG His Thr (H) (T) Gln GUU GCU GAU Asp GAÇ GGU (Q) lle Arg (R) (1) GUC Val GUA GCC Ala GCA GGC Gly GGA GAA Glu OGAG GUG GCG GGG O 1 3 4 First mRNA base (5' end of codon) - U A GUca Gbc AG Third mRNA base (3' end of codon)What is the one-letter amino acid sequence formed from the following mRNA that codes for a pentapeptide that is an endorphin called Leu-enkephalin? 5' AUG - UAC - GGU - GGA - UUU - CUA - UAA 3'
- The following is a section of a messenger RNA: 5' GACUACAUA 3' Look at the sequence carefully and using the following genetic code, translate the MRNA into an amino acid sequence. Second Letter U A G Phe uCu UC | Tyr UGU Cys u UUU U UUC UUA UUG UAU UAC UAA UAG UGC Stop UGA Stop A Stop UGG Trp G Ser UCA UCG Leu CAU CAC САА CAG CU CU His CGU U c Cuc CUA CUG Leu cc CCA CG CGC Arg CGA CG Pro Gin A 3rd G 1st letter AUU Ser u letter ACU lle ACC ACA AAU Asn AGU Thr AAC AAA A AUC AGC AUA AGA | Lys A Arg G AUG Met ACG AAG AGG Asp GUU G GUC GUA GCU GAU GAC GAA GGU GGC Gly Val |GCC GCA GCG Ala GGA GGG A Glu GUG GAG G O Tyr-Val-Val O Asp-Tyr-lle O Leu-Met-Tyr O lle-His-Gln O Asp-Thr-Leu-Tyr-Thr-His-lle 星w/opCulGACU GAC UC 4C According to the Genetic Code Sheet below, which of the following amino acid sequences corresponds to this MRNA strand? CỤC AAG UGC UUC PHE GLU ASP SER ALA TYR U A STOP A GU VAL U CIS U U G A STOP IG TRP ARG AC U LEU SER UG PRO ASN HIS THE GLN MET ILE ARG O a lys-leu-cys-phe O b glu-cys-pro-phe leu-lys-cys-phe O d leu-glu-leu-val U...pdfWhich of the following polypeptides (written from their N terminal ends) is encoded by the following mRNA? (hint: there is no left or write in a cell!) 3' GG AUG AGU AUA CUU ACG GGU GAA GUA CAU UAA CCC C5' O O O Met Lys Trp Ala Phe lle Met Ser lle Leu Thr Gly Glu Val His Tyr Lys Arg Thr Phe Tyr Met none of the above His Val Glu Gly Thr Leu lle Ser Met
- 1. Using the genetic code, determine the sequence of amino acids encoded by the mRNA codocn sequence 5' GCC-AUG-GUA-AAA-UGC-GAC-CCC 3' 2. Using the genetic code, determine the sequence of amino acids encoded by the mRNA codocn sequence 5' CAU-CCU-CAC-ACU-GUU-UGU-UGG 3'A fragment of a polypeptide, Met-Thr-Ile-Ser-Asp-Ile is encoded by the following sequence of DNA:Strand A - TACGATGACGATAAGCGACATAGC - Strand B - ATGCTACTGCTATTCGCTGTATCG -Which is the transcribed (template) strand? Write the sequence of the resulting mRNA transcript. Add labels to the strands above to show the 3’ and 5’ ends.Translate the following mRNA: 5-A U G A A A U U U C U U U A G G U C G A A -3 NH3+- Met-Leu-Phe-Val- COO- NH3+- Met-Thr-Val-Ser- COO- NH3+- Met-Glu-Gln-Ser- COO- NH3+- Met-Asp-Ser-Pro- COO- NH3+- Met-Lys-Phe-Leu- COO-
- Here is the nucleotide sequence of an imaginary strand of DNA: 5’ AATTGGCCATGC 3’. If this strand of DNA was transcribed, the resulting messenger mRNA molecule would be: Met-Val-Tyr-Lys 3’ TACGTACG 5’ 3’ TTAACCGGTACG 5’ 3’ UUAACCGGUACG 5’1. Given is the 30 nucleotides in the human gene for hemoglobin (the oxygen-carrying protein in the red blood cells): 3’ TAC-CAC-GTG-GAC-TGA-GGA-CTC-CTC-TTC-AGA 5’ a. What is the amino acid sequence based on this mRNA? b. A very important mutation in human hemoglobin occurs in this DNA sequence, where the T at nucleotide 20 is replace with an A. The mutant hemoglobin is called sickle cell hemoglobin and is associated with severe anemia. What is the amino acid replacement that results in sickle-cell hemoglobin?UCUGAUGGGCUUU... Beginning with the Start codon (for Met), which amino acids, in order, are coded for by this section of mRNA? Second letter threonine, methionine, glycine methionine, glycine, phenylalanine methionine, valine, glycine, phenylalanine serine, aspartic acid, glycine, leucine First letter G U UUU phe UUC. UUA UUG CUU CUC CUA CUG Leu Leu GUU GUC GUA GUG AUU AUC lle ACU ACC AUA ACA AUG Met ACG UCU UCC UCA UCG Val CCU CCC CCA CCG GCU GCC GCA GCG Ser Pro Thr Ala CAU CACJ CAA CAG UAU UGU UGC Tyr Cys UAC UAA Stop UGA Stop A UAG Stop UGG Trp G His Gln AAU Asn AAC AAA AAG Lys GAUT GAC GAAT GAG G CGU CGC CGA CGG AGU 1 Ser AGC AGA AGG GGU Asp GGC GGA Glu GGG Arg Arg DUAG DUAU DUAG ULAG Third letter Gly