Question 8 Question 8ListenA bacterial cell divides in the presence 32P, which is a radioactive isotope ofphosphorous. This atom becomes part of the phosphate which is in DNA. After thefirst division, the bacteria is switched into a growth medium in which thephosphorous is not radioactive and is kept in this media for the rest of theexperiment. The bacterial cell divides until there are 8 cells. How many of these cellswould be radioactive if DNA replication is conservative, not semi-conservative?a) Ob) 1○ c) 2d) 4e) 8

In conservative DNA replication both the strands of DNA of the parent cell become part of one of the…

Answered: A bacterial cell divides in the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 17 What is the nucleotide sequence of the DNA strand that is complementary to 5'-ATCGCAACTGTCACTA-3 A 5'-UAGUGACAGUUGCGAU-3' B 5-TAGCGTTGACAGTGATA-3 5'-ATCACTGTCAACGCTA-3 D 5'-TAGTGACAGTTGCGAT-3'
QUESTION 14 The Hershey and Chase experiments, in which radioactive phosphorus (32P) and radioactive sulfur (35S) were used, demonstrated that O a. DNA labeled with 32P is transferred from the bacteriophage to the virus O b. DNA may be the hereditary material, although bacteriophages transfer both DNA and proteins into their hosts O C. proteins labeled with 35S become deactivated and unable to be transferred O d. DNA labeled with 35S and proteins labeled with 32P can be traced over the course of an experiment O e. bacteriophages transfer their DNA, not their coat proteins, into their hosts QUESTION 15 Which of the following makes its own food using energy and simple raw materials it obtains from nonbiological sources? O a. Cell O b. Producer O C. Protist O d. Consumer O e. Nutrient QUESTION 16 A salt will dissolve in water to form O a. bases O b. acids O C. buffers O d. jons other than H* and OH" O e. only hydrogen and oxygen bonds
QUESTION 14 The Hershey and Chase experiments, in which radioactive phosphorus (32P) and radioactive sulfur (35S) were used, demonstrated that Oa. DNA labeled with 32P is transferred from the bacteriophage to the virus O b.DNA may be the hereditary material, although bacteriophages transfer both DNA and proteins into their hosts Oc. proteins labeled with 35S become deactivated and unable to be transferred O d. DNA labeled with 35S and proteins labeled with 32P can be traced over the course of an experiment O e. bacteriophages transfer their DNA, not their coat proteins, into their hosts QUESTION 15 Which of the following makes its own food using energy and simple raw materials it obtains from nonbiological sources? O a. Cell O b. Producer O C. Protist O d. Consumer O e. Nutrient QUESTION 16 A salt will dissolve in water to form O a bases O b.acids O c. buffers O d. jons other than H* and OH O e.only hydrogen and oxygen bonds QUESTION 17 The solvent, cohesive, and temperature…
QUESTION 5 the heteroduplex region is a region that any DNA that is double stranded O DNA contains 2 strands of DNA, one of maternal and one of paternal origin O when 2 identical DNA sequences pair up
Question 21 (1 point) Based on what we learned, what is true about E. Coli DNA replication? O The polymerase activity of both DNA polymerase I and II is 5' 3". O DNA polymerase lII has a lower processivity than DNA polymerase I. O For the leading strand synthesis, the DNA polymerase needs to keep loading new B-sliding clamps. DNA synthesis is continuous on the lagging strand and discontinuous on the leading strand. DNA polymerase III does not require a primer to start DNA replication. Question 22 (1 point) Below which describes the regulation of the lac operon transcription? All Match Case Match Diacritics Whole Words 1 of 1 match
Question 21 Your research team has been tasked with using a variety of horizontal gene transfer techniques to determine the overall order of genes on the bacterial chromosome in the R. ibiscus prokaryote usin, growth of bacteria. Growth in media is the byproduct of cell division and thus DNA replication precedes any kind of growth that we might observe. Select the statement below that is true of prokaryotic DNA replication. O it requires only DNA polymerase; no primase is necessary O it begins at multiple origins of replication throughout the bacterial chromosome O it begins at one origin of replication on the bacterial chromosome telomerase is needed to break the hydrogen bonds between DNA strands Question 22 Now that we've got that settled; let's start looking at some data. You decided to begin with some interrupted mating experiments to determine chromosomal location of various genes that encode biological factors necessary for survival. You began with a donor Hfr R. ibiscus that…
QUESTION 9 The function of DNA polymerase in the process of DNA replication is O a. to prevent the single-stranded DNA re-forming into double-stranded DNA before replication can occur. b. to catalyse the addition of nucleotides O c. to bind to the double-stranded DNA molecule and open it to form single strands
Question 75 You have isolated a small part of the DNA of a paramecium. The following diagram shows part of the template strand base sequence of the DNA molecule. T-T-T- A - C-G-C-A-T-A–T The coding DNA strand bonded to the DNA template strand illustrated above would have this sequence: O AAATGCGTATA O AAATGCGTATA O AAATATGCGTA O AAACGTATGCGC
Question 8 Electrophoresis in agarose gel |A The DNA will migrate to the negative elec- trode due to the presence of phosphate groups. B For a given DNA molecule, the higher the percentage in G/C pairs, the less the DNA will migrate. C The migration speed depends on the agarose percentage. D] The migration of an undigested, circular plasmid DNA will depend solely on its number of base pairs. E If the migration buffer is at physiological pH (7.4), the DNA will not migrate.
QUESTION 10 The drug shown in the figure below is an anticancer agent that disrupts the overall chromatin structure by binding AT-region regions of DNA and preventing the interactions shown in the figure below. The region A site shown below are called [x]? H OH HN HN HO NH Region A? AT-specific drug cooooo Nuclear matrix Transcription/replication machinery
Question 5 The plasmid PSU922 is a circular DNA containing 25000 base pairs. The ß-gal gene codes for the enzyme ß-galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp gene confers ampicillin resistance. EcoRI Bam HI BamHI HindIII PSU922 BamHI Bam HI EcoRI ori C Referring to the plasmid above, which restriction site is best for inserting a DNA fragment for analysis? (A) BamHI (B) Hindill (C) ECORI (D) any of the enzymes will do AmpR F-gai
Question 5 DNA polymerase: |A Is able to open the helix at the origin of replication. B Works on both strands during replication. C Synthesizes phosphodiester bonds between the two strands of the DNA helix. D] Is an enzyme that recognizes the gene pro- moter. E Is responsible for the transcription.

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