Electrophoresis of a mixture of lysine, histidine, and cysteine is carried out at pH 7.64. Describe the behavior of each amino acid under these conditions.

Q: calculate the % yield of preparation of 2-aminobenzoic acid: mass of phthaliamide = 2.95g mass of…

A: In the  question two mass of 2-aminobenzoic acid is given. Hence, the individual percent yield is…

Q: In a solution at pH 7.0, a polymer of lysine( polylysine) is a random coil with no secondary…

A: Polylysine forms a random coil at low pH , alpha helix at a pH above 10.6 and gets transformed into…

Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated bypaper…

A: As multiple questions are mentioned here, so the solution to the first part is given here. Paper…

Q: You have the following polypeptides at pH 7.25 and are attempting to separate them using an anion…

A: For an amino acid residue,    If pKa > pH, the ionizable group remains in its protonated state.…

Q: Describe the physical properties of the polymer including appearance and color. If nylon-6,6 was not…

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Q: Give the functions of the following ingredients, then name a branded/commercial skin or hair care…

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Q: The electrospray ionization mass spectrum of the tripeptide Ala-Met-Thr shows peaks of…

A: Given peptide is: Ala-Met-Thr

Q: Fig. 1 shows the possible effect of pH on recovery and impurity removal at the selection of a…

A: The Y-axis of the graph represents the percent recovery of the impurity, and the X-axis of the graph…

Q: How would the pKa values of carboxylicacids, alcohols, ammonium ions 1RNH32 + , phenol, and an…

A: The strength of a chemical substance acidity is determined by its pKa value.

Q: The catalytic activity of an enzyme is being measured at pH 7.5 and requires that the side chain of…

A: The enzymes worked as a catalyst in the biological reaction. These can be present in the active or…

Q: The isoelectric point, pI, of the protein trypsin is 10.5, while that of horse liver alcohol…

A: The isoelectric point of amino acid is the pH at which the net electronic charge of the amino acid…

Q: Calculate the standard free-energy changes of the following metabolically important enzyme-…

A: a.

Q: You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 6.50.…

A: Buffers are solutions that are used in experiments to resist the change in pH. Carbonic acid is a…

Q: The UV-vis absorption spectrum of the peptide RYYFE collected in a 1 cm pathlength cuvette exhibits…

A: the optical path length and the molar concentration of the solution. i.e.…

Q: You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 10.50.…

A: Buffer Solution is a water solvent based solution which consists of a mixture containing a weak acid…

Q: Outline the possible reaction pathways with proper mechanism for the formation of Nylon 6 from…

A: Polymerization:  It is a chemical process when a monomer (single unit) combined through various…

Q: Rank the amino acids used in the experiment in Part II in the order of increasing solubility in…

A: The solubility of amino acids, generally, depending upon the nature of the side chain and they are…

Q: Explain the order of elution (with a buffer of pH 4) of the following pairs of amino acids through a…

A: (a)  Comparing the structure of aspartate and serine as shown. Aspartate has the carbonyl group…

Q: Which of the following tetrapeptides will be retained on a cation-exchange column at pH 8.2? O HGTH…

A: Ion exchange chromatography separates ions and molecules based on their net overall surface charge.…

Q: Which do you expect to have a greater binding constant (equilibrium constant for binding to the…

A: Ethylene diamine (en) is bidentate chelating ligand in which two nitrogen bind the metal ion and…

Q: Consider an amino acid with the following pKa values: pKa1 = 2.71 and pK, = 9.31 At what pH value in…

A: Amino acids are amphoteric in nature due to the presence their ionizable α-amino and α-carboxylic…

Q: At pH 5.0? Q2) (a) Draw the structure of the predominant form (principal species) of…

A:

Q: Deduce expressions for the fractions of each type of species present in an aqueous solution of…

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Q: The electrophoresis of a sample containing 2-amino-4-methylpentanoic acid. (pKa of a-COOH = 2.2, pKa…

A:

Q: The pKa values of the conjugate acids of 2-aminopyridine (6.71) and 4- aminopyridine (9.11) are as…

A: It is given that 2-amino pyridine conjugate acid has a pKa value of 6.71 and 4-aminopyridine…

Q: Suppose two phosphate groups in DNA (each with a charge of 21) are separated by 12 Å. What is the…

A: The expression of electrostatic energy is shown below: Where; q1 = quantity of charge on object 1…

Q: Consider a histidine residue within a protein. Based on the histidine side-chain pKa value of 6.0…

A: Histidine is a basic amino acid used to make up proteins which contain an amino group and a…

Q: 3. Show the reaction between aqueous hydrochloric acid and alanine. Define the equilibrium constant.

A: Amino acids have the following general formula: Different amino acids have different R group,…

Q: what is pseudo order treatment

A: Pseudo is Latin word which means ‘fake’. A Pseudo order reaction can be defined as a bimolecular…

Q: Use information from terminal residue analysis and partial hydrolysis to determine thestructure of…

A: An amino acid is a hydrocarbon that contains a carboxylic acid group, amino group along with R group…

Q: What are the net charges of the following five-amino-acids peptides at pH 14.0 RRKKQ? (please…

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Q: All methyl esters of long-chain aliphatic acids (e.g., methyl tetradecanoate, C13H27COOCH3) show…

A:

Q: Determine the buffer capacity of Alanine and Histidine at pH 2.0 and 6.0 respectively and discuss…

A: For the alanine, the value for pKa is 2.34. Now the salt to amino acid ratio to achieve the pH of…

Q: The pka of ascorbic acid is 4.2 at 24C. At what pH is the ratio of the deprotonated form to the…

A: Henderson Hasselbach equation is pH=pKa+log[deprotonated form][protonated form]

Q: If prior to the analysis, the protein X needs to be solubilised in 250 mM acetate buffer, pH 4.0 to…

A:

Q: The amino acid leucine has an Rf value of 0.73 and arginine has an Rf value of 0.20.  Draw their…

A: Rf value is the ratio of distance travel by the solute to the distance travel by solvent from base…

Q: what is The method that would be BEST to use to monitor protein secondary structure during the…

A: The secondary structure of the protein is affected by the complex folding properties of the protein…

Q: Rank the migration rate of the following amino acids in ascending order towards  the cathode when…

A: PI stands for isoelectric point. If pH is less than isoelectric point then amino acid will move…

Q: The isoelectric point of tyrosine is 5.63. Toward which electrode does tyrosine migrate on paper…

A: Amino acid are the substances which have an amine group, a carboxyl group and a side chain which is…

Q: One of the side chains in this molecule would be charged at a pH of 7.3. Locate the side chain, and…

A: A group gets positively charged when its pKa is greater than the given pH A group gets negatively…

Q: How does the shape of a titration curve confirm the fact that the pH region of greatest buffering…

A: As there are three question are present I answered only one. you can repost your remaining two…

Q: 1. Write an equation for the oxidation of glucose by CuO if the compound is oxidized completely to…

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: The velocity v with which a protein moves through water under the influence of an electric field…

A: The given expression is, The value of a is 0.80 . The value of b is -4.0×10-3. The value of c is…

Q: Asparagine and glutamine residues in proteins are deamidated in an aqueous solution to yield…

A: Deamidation is the conversion of amide group to another functional group. Side chain of amino acid…

Q: If you had a mixture of proteins with different sizes, shapes, and charged and you separated them…

A: Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are…

Q: Name four factors (bonds or other forces) that contribute to stabilizing the native structure of a…

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Q: d. Calculate the Gibbs energy and the equilibrium constant for the denaturation of chymotrypsin at…

A: Protein will be more stable in that form which is having more concentration at equilibrium.

Q: Electrophoresis at pH 7.0 of the following lipid mixture is perfo lipid mixture…

A: