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3. Why can there be no generalized media that can support all types of microorganisms? Briefly justify in less than 50 words.
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- 1. Table 1 lists a typical recipe for growing bacteria in the lab. A researcher discovered a potentially new species of bacterium from a soil sample and attempted to grow it in this media. Unfortunately, the bacterium did not grow. Identify at least three components that you suggest adding to the medium to enable growth. Provide a reason for adding each component. Table 1. per 1000 mL 1g Bacteria culture media NazHPO4•7H2O KH2PO4 3g 5 g 1 g 0.4% (wt/vol) 0.2% (wt/vol) NaCl NHẠC1 Glucose Casamino acids1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.9. Is the eosin methylene blue agar (EMB) a complex or defined medium? Explain based on its composition. Is MSA a A) differential, B) selective, or C) both differential and selective media? Explain based on what kind of microorganisms it allows to grow.
- 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant. Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. 200%- 927 PM 0 日 2/7/2021 21 DELL Insert Delete F11 F12 PrtScr F9 F10 F7 F8 F6 F3 F4 F5 Esc F1 F2 Backspace & # 2$ 8 4 1 2 P Home R T E Enter K Shift V B Page Down Page Up Alt Ctrl Alt THome ノ/ *00 Σ1. What kinds of media would be used to culture and Identify this microbe?2. What are some other potential microbes that could have this infection?
- 8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. F10 F12 F6 %24 6. R. Home Enter K F G A Shift C Page Up Page Down Alt Ctrl Alt Σ1. If you are a thermophilic endospore forming bacterium what would be the appropriate and economically wise method for your preservation? Which culture collection centers will you select and why?1. why is there a need to incubate cultures at room temperature? How can this affect microbes? 2. Explain why an inverted image is seen under a compound microscope.
- 1. What is the appropriate and economically wise preservation method for a thermophilic endospore forming bacterium? Discuss and explain it comprehensively, and please, do not just copy from somewhere. Choose among this methods: periodic transfer to fresh media, overlaying cultures with mineral oil, freeze-drying, cryopreservation, drying, sterile distilled water7a. A petri plate is given to you with 80 colonies on it. I tell you that I did five 10x dilutions and then plated 0.1 ml from my last tube to get 80 colonies on a plate. How many living bacteria per ml were in that original sample? Draw out (or describe) all of the dilutions I would have done to get this number. 7b. Again, I have a plate with 80 colonies on it, but I tell you that I did one 100x dilution and two 10x dilutions before I plated that 0.1ml from my last tube. How many living bacteria per ml were in that original sample? Draw out (or describe) all of the dilutions I would have done to get this number. please include drawings. thank youWhich of the following is NOT correctly matched? A. Halophile – microbe that can grow in an environment with high osmotic pressure (e.g., salt) B. None of the other four answers (all are correctly matched) C. Agar – complex polysaccharide from seaweed used as a solidifying agent in culture media D. Colony – a population of microbial cells arising from a single cell or spore or from a group of attached cells E. Capnophile – microbe requiring an elevated molecular oxygen (O2) environment for optimal growth