1. What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame? 2. Why is heat a highly effective sterilizing agent? 3. Which of the three methods of heat sterilization is the most appropriate and practical to use in sterilizing antibiotic solutions? (Direct flame, Dry heat, Moist heat)

Q: Briefly define culture medium.

A: Microbiology is the branch of science which deals with the study of microorganisms like, bacteria,…

Q: What type of medium is used to maintain and preserve specimens before clinical analysis? a.…

A: Microorganisms are small, unicellular, and microscopic organisms. Any organism capable of causing…

Q: Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0…

A: Please follow step 2 & 3 for detailed explanation.

Q: 1. TRUE or FALSE. An autoclave can be used to sterilize growth media?

A: Growth media are solid, liquid or semisolid mediums which contains nutrients to support the growth…

Q: 1. Describe and give the principle of each equipment used for sterilization in microbiology. (refer…

A: Sterilisation refers to any process that removes, kills or deactivates all forms of life and other…

Q: Blood agar media  is a _________.   Selective media Differential media Rich media Enriched media…

A: A medium can be defined as gel or liquid which helps in growth of a bacterial culture. Medium is…

Q: Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: Why can there be no generalized media that can support all types of microorganisms? Briefly justify…

A: The first bacterial culture media were broths made by infusion or enzymatic digestion of assorted…

Q: 2. The following is an incomplete schematic diagram for the isolation procedure used. Label each…

A: Isolation of Micro organisms -- Introduction --The term isolation is defined as separation of a…

Q: 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating…

A: Agar is formed by two components, agarose, and agaropectin molecules. Agar is used as culture media…

Q: The mechanical removal of microbes from a limited area of living tissue is referred to as -- a.…

A: Control of microbial propagation is very significant in medical, pharmaceutical, food industries,…

Q: 8- The following are methods of sterilization EXCEPT: O c) Sterilization by filters O b) Autoclaving…

A: Introduction: A combination of heat, chemicals, irradiation, high pressure, and filtration can be…

Q: Why do you disinfect your work area at the beginning of lab and then again at the end of lab?   2.…

A: Pure culture - In microbiology if  one organisms cultured in midium. It is obtained from mixed…

Q: Which of the following are proper disinfection steps? Check all the O Remove organic matter O…

A: Introduction: Disinfection is substances that are applied to non-living objects to destroy…

Q: Bacteria are not used as probiotic agents. True O False The growth of bacteria that form endospores…

A: Bacteria are prokaryotic microorganisms. Bacteria are ubiquitous in nature and are found almost in…

Q: 2) Finish the following chart for the data obtained for the effect of disinfectants on Escherichia…

A: As per our company guideline we are supposed to answer only first question or first 3 sub parts of…

Q: 1. What is the difference between quadrant streak method A from method B? 2.  Why do we use a…

A: Introduction :  Microbiology is the branch of biology that specialises in studying microbial life.…

Q: The most versatile method of sterilizing heat-sensitive liquids isa. UV radiation b. exposure to…

A: Sterilization is a process of removing or killing or deactivating mostly all forms of life present,…

Q: For each item on the following list, propose a reasonable method ofsterilization. You cannot use the…

A: There are several sterilization techniques which can be used. But, the technique to be used varies…

Q: 3. Give specific examples of media which are sterilized by the following method: a. Boiling or…

A: Sterilization is the process of destruction of all forms of microorganisms from various substances.…

Q: 1. While working with Mannitol Salt Agar you notice the growth of staphylococci however other…

A: Mannitol Salt Agar is a selective and differential media used in microbiology.

Q: Transparent double-sided dishes used for growing microbes are most commonly called A) Petri dishes.…

A: The micro organisms are grown in specific containments for identification of the characteristic…

Q: "Sterilisation is defined as the process by which all-living cells, viable, spores and virus are…

A:

Q: 9. Complete the table by transcribing the following culture media, classify as to…

A: Introduction :  A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: 3. For each category of microbial control, list examples. Sterilization Disinfection Sanitization/…

A: All of these are methods are used to prevent infection by slowing or stopping the growth and spread…

Q: Spread plating_is used to sterilize the spreader or Lrod: a. Ethanol bHeating over flame C.…

A: Spread plate technique  is used to isolate bacteria . It is a microbiological technique .

Q: 5. Provided with the following data, compute the corresponding CFU/ml of the original culture.…

A: One of the techniques in microbiology for culturing the microbes is the spread plate technique. The…

Q: 4. Use the words presented in below to create a concept map illustrating the relationships among…

A: All these terms mentioned in the question, are related to the isolation, cultivation, and…

Q: 0.22 micrometers

A: The size of bacteria generarally ranges from 0.2 to 5 micrometer, therefore, most of the bacteria…

Q: which of the following is a limitation of the autoclave? a. long length of time required for…

A: Sterilization is the process which helps in removal ,killing and deactivation of all kinds of life…

Q: 1. Can culture media be sterilized in the hot air sterilizer? Why or why not?

A: As per the honor code, we answer only one question at a time therefore we are answering the first…

Q: Distinguish between the term’s sterilization, disinfection, antiseptics, and decontamination.…

A: These all are the method of killing or reducing the number pathogenic microorganism but these terms…

Q: 1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C.…

A: The removal of any microorganism or viable spores or bacteria or viruses from the surface. The…

Q: 1. Give the principle involved in the operation of the following instruments and their specific…

A: Any method that removes, kills, or deactivates all forms of life is referred to as sterilization (in…

Q: All of the following can achieve sterilization (killing endospores, but not prions) EXCEPT gamma…

A: 9 - option B -autoclaving at 121 degree celsius for 25 min,15 psi As per the guidelines,I can answer…

Q: Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big…

A: Petri plates, slants and stabs of Culture media such as Luria Bertani agar is used in microbiology…

Q: 1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or…

A: 1. A soil-inoculated broth cannot be considered as a “pure culture”. A pure culture is a collection…

Q: The technique used in bacteria for endospore staining can also be used to observe fungal spores.…

A: *Endospores staining is used to recognize presence of spore in bacterial cells This needs staining…

Q: Explain shortly the concept of differential centrifugation and how you would limit pellet…

A: Centrifugation is a mechanical technique. It helps to separate mixtures based on their size, shape,…

Q: Which of the following is used in the sterilization of the medium? a) benmarge b) swab c) autoclave…

A: Sterilization is the process of making something (instrument, swab, room etc. ) free of microbes…

Q: What type of medium is best used to maintain a preserve specimen before clinical analysis? A.…

A: A growth or culture medium is solid, liquid or semi solid in nature that is used to support the…

Q: 1. In an autoclave, how are temperatures above boiling achieved?(short answer) 2.. Can culture…

A: Culture media  Culture media is a growth media of microorganisms like bacteria it may be solid,…

Q: The following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii.…

A: Sterilization: Microorganisms cause contamination, infection, decay and it is important to remove…

Q: 1. What is one advantage of utilizing the pour plate technique over the streak plate technique ?

A: Answer: THERE ARE THREE TYPES OF CULTURE METHODS  1. POUR PLATE TECHNIQUE 2. STREAK PLATE TECHNIQUE…

Q: Which of the following is a limitation of the autoclave? a)  Long length of time required for…

A: Answer- In research the use of contamination free equipments is mandatory. The main apparatus used…

Q: 5. Provided with the following data, compute the corresponding CFU/ml of the original culture.…

A: Introduction For CFU count the average no. of colonies chosen is 30-300. Above this range, the…

Q: . Why not test water samples directly for Salmonella typhosa or other pathogens?

A: Salmonella Typhi bacteria infect the blood and intestine of humans. The disease caused by this…

Q: B. Match the following. A. Killing all bacteria on medical equipment via autoclaving 1. Sanitization…

A: Sterilization methods involve all those methods that aim to remove all the live, dead cells or…