Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Question
Chapter 15, Problem 17Q
Summary Introduction
To enlist:
Some advantages to the use of HIV testing for patient antibody to virus rather than the virus itself.
Concept introduction:
The antibodies are produced by immune responses against antigens and antigens bind to antibodies tightly. The specificity of antibodies towards different antigens is used in immunological techniques.
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Discuss, with examples, the advantages and limitations of testing for viral protein/nucleic acid and testing for antibody to the virus as diagnostic tests for a virus infection.
why do we have to do a western blot confirmatory testing after doing an ELISA screening test for HIV?
What are advantages and disadvantages of the antibody test with the coronavirus?
Chapter 15 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 15.1 - Prob. 1AYPCh. 15.1 - Provide a one-sentence description for each of...Ch. 15.2 - Identify factors that may affect the...Ch. 15.2 - Prob. 4AYPCh. 15.2 - NCLEX PREX 1. An RN is training a new staff nurse...Ch. 15.2 - NCLEX PREX 2. A clinical form used to report data...Ch. 15.3 - List at least three different tests that fall in...Ch. 15.3 - Prob. 6AYPCh. 15.3 - Discuss two major drawbacks of phenotypic testing...Ch. 15.3 - Q. What technique in this chapter do most home...
Ch. 15.3 - NCLEX PREX 3. When determining the clinical...Ch. 15.4 - Define the term serology, and explain the...Ch. 15.4 - Identify two immunological diagnostic techniques...Ch. 15.4 - Prob. 2MMCh. 15.5 - Explain why PCR is useful for infectious disease...Ch. 15.5 - Name two examples of techniques that employ...Ch. 15.5 - Explain how whole-genome sequencing can be used...Ch. 15.5 - Prob. 13AYPCh. 15.5 - Prob. 3MMCh. 15.6 - Describe the benefits of lab on a chip...Ch. 15.6 - Prob. 15AYPCh. 15 - When using pulsed-field gel electrophoresis,...Ch. 15 - Explain why it is possible to identify some...Ch. 15 - Serotyping identifies distinct members of the same...Ch. 15 - Prob. 4QCh. 15 - Name some bacterial structures that might be...Ch. 15 - You perform a lumbar puncture on a patient with...Ch. 15 - Which category of diagnosis is represented by...Ch. 15 - Write a paragraph that explains the mycobacterial...Ch. 15 - You inoculated a biochemical test strip with a...Ch. 15 - Prob. 10QCh. 15 - You perform a Kirby-Bauer disk diffusion test to...Ch. 15 - Why might culture conditions affect the results of...Ch. 15 - Which of the following techniques is most likely...Ch. 15 - Why is it more important to use selective media...Ch. 15 - What type of diagnostic method do you think would...Ch. 15 - T or F: Bacterial infection causes the expression...Ch. 15 - Prob. 17QCh. 15 - Prob. 18QCh. 15 - Prob. 19QCh. 15 - When PCR is performed by hand (not with a...Ch. 15 - What kind of a control would be important to run...
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Similar questions
- Discuss in detail, how you would produce recombinant protein subunit vaccines for Influenza virus.arrow_forwardEIAs are extremely sensitive diagnostic tools. Why,then, is the immunoblot (Western blot) procedure usedto confirm screening tests that are positive for humanimmunodeficiency virus (HIV)?arrow_forwardExplain how a drug that blocks DNA recombination would affect the life cycle of HIV. Be as specific as possible.arrow_forward
- Which of the following assays would utilize monoclonal antibodies? MRI imaging of mice expressing viral constructs that express bioluminescence euthanization of mice infected with engineered viruses that express reporter proteins and staining for protein expression euthanization of SCID mice followed by dissection and flow cytometry euthanization followed by dissection and assaying for the virus in tissuesarrow_forwardAntiretroviral drugs target various aspects of HIV replication and infection. Which of the following enzymes are targets of antiretroviral drugs? Select ALL that applya).reverse transcriptaseb)integrasec).ligased).proteasee).DNA polymerasearrow_forwardGive an example of a viral diagnostic method that detects viral nucleic acid and one that detects viral protein with explanationarrow_forward
- The plaque assay plate below was made from a dilution of 10-7 and 0.1 ml of the dilution was plated on the cell monolayer. What is the virus titer in PFU/ml? Note: 10e1 means 10 to the first power, etc. Select all answers that apply.arrow_forwardIn our pandemic world, COVID testing has become a very common thing (unfortunately necessary). When receiving a test to detect the COVID virus, a person may or may not have COVID, and the test result could come back either positive or negative. (a) Explain what specificity means in the context of a COVID test. (b) Explain what sensitivity means in the context of a COVID test.arrow_forwardWhat are some of the challenges of using recombinant molecules to treatment of HIV?arrow_forward
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