Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 15, Problem 20Q
When PCR is performed “by hand” (not with a prepackaged test kit), the lab environment has to be scrupulously clean. Explain why that might be.
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PCR technique is based on DNA transcription.
True
False
sample containing at least one molecule of DNA serves as the starting material for PCR.
True
False
A student is trying to add 9.0 pmol of primer mix to a 20.0 µL PCR. The primer mix is at a concentration of 50.0 µM, and the student determines that a serial dilution is required because directly adding the primer mix would require a volume less than 1.00 µL. The student takes 1.40 µL of the primer mix stock and mixes it with 25.0 µL of ultrapure H2O. Calculate the volume (in µL) of the intermediate solution required to add the correct amount of primer mix to the PCR.
Chapter 15 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 15.1 - Prob. 1AYPCh. 15.1 - Provide a one-sentence description for each of...Ch. 15.2 - Identify factors that may affect the...Ch. 15.2 - Prob. 4AYPCh. 15.2 - NCLEX PREX 1. An RN is training a new staff nurse...Ch. 15.2 - NCLEX PREX 2. A clinical form used to report data...Ch. 15.3 - List at least three different tests that fall in...Ch. 15.3 - Prob. 6AYPCh. 15.3 - Discuss two major drawbacks of phenotypic testing...Ch. 15.3 - Q. What technique in this chapter do most home...
Ch. 15.3 - NCLEX PREX 3. When determining the clinical...Ch. 15.4 - Define the term serology, and explain the...Ch. 15.4 - Identify two immunological diagnostic techniques...Ch. 15.4 - Prob. 2MMCh. 15.5 - Explain why PCR is useful for infectious disease...Ch. 15.5 - Name two examples of techniques that employ...Ch. 15.5 - Explain how whole-genome sequencing can be used...Ch. 15.5 - Prob. 13AYPCh. 15.5 - Prob. 3MMCh. 15.6 - Describe the benefits of lab on a chip...Ch. 15.6 - Prob. 15AYPCh. 15 - When using pulsed-field gel electrophoresis,...Ch. 15 - Explain why it is possible to identify some...Ch. 15 - Serotyping identifies distinct members of the same...Ch. 15 - Prob. 4QCh. 15 - Name some bacterial structures that might be...Ch. 15 - You perform a lumbar puncture on a patient with...Ch. 15 - Which category of diagnosis is represented by...Ch. 15 - Write a paragraph that explains the mycobacterial...Ch. 15 - You inoculated a biochemical test strip with a...Ch. 15 - Prob. 10QCh. 15 - You perform a Kirby-Bauer disk diffusion test to...Ch. 15 - Why might culture conditions affect the results of...Ch. 15 - Which of the following techniques is most likely...Ch. 15 - Why is it more important to use selective media...Ch. 15 - What type of diagnostic method do you think would...Ch. 15 - T or F: Bacterial infection causes the expression...Ch. 15 - Prob. 17QCh. 15 - Prob. 18QCh. 15 - Prob. 19QCh. 15 - When PCR is performed by hand (not with a...Ch. 15 - What kind of a control would be important to run...
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- Why is it important to wear gloves when setting up the PCR tubes?arrow_forwardWhy does the temperature constantly fluctuate inside the PCR machine? What is the purpose of the 95oC temperature? 60oC? 72oC? (Give answers longer than a single word.)arrow_forwardIdentify if the following statements are true or false. Quantitative PCR is also known as the real-time PCR Real-time PCR method uses fluorescent dyes such as acridine orangearrow_forward
- Which of the following ARE part of a typical PCR reaction mixture? DNA ligase dNTPs (mix of nucleoside tri-phosphates) RNA primers made by primase enzyme template DNA, often from cells collected from hair, cheek swab, or blood 2 DNA primers polymerase enzymearrow_forwardBrenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break? A. Obtaining and handling DNA sample without wearing googles or gloves B. Improper use of human DNA samples C. Violating Patient Confidentiality D. Disposing of bio hazardous material in a regular trasharrow_forwardThe primers used in PCR are double stranded DNA oligosaccharides. True Falsearrow_forward
- In a conventional PCR reaction cycle, which of the following step is NOT needed for a successful PCR reaction? anneal amplification refrigeration O denaturearrow_forwardPlease answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardState the five basic steps of DNA fingerprinting using the RFLP method. Why do you think the PCR method is of more use in crime scene investigations?arrow_forward
- The production in a pcr reaction is: nucleotids double strand circular dna double stranf linear dna single steand amyisense rnaarrow_forwardIf the RT-PCR test is negative, give instances when repeat RT-PCR test should be done. If repeated, how many days after the first test should the 2nd test be done?arrow_forwardYou receive a tube containing 18.8 nmol of lyophilized (freeze dried) primers to be used in PCR. How many µL of water would you need to add to create a 100 µM solution?arrow_forward
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