Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 15, Problem 4Q
Summary Introduction
Introduction:
Mass spectroscopy provides a reliable molecular weight for an unknown compound and also gives us the information about the molecular formula as well. Fragmentation gives the resonance-stabilized cations whenever possible. Loss of a small molecule is usually indicated by a fragment peak with having an even mass number corresponding to loss of an even mass number. Fragmentation produces the most stable carbocation and radicals when occurs in
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Mass spectrometry identifies microbes via a. fluorescence of antibodies. b. precipitation reactions. c. protein fingerprints. d. fluorescence of antigens.
A. What are the 4 “Koch’s Postulates”?
B. Are they still valid today? How would we use them to establish the relationship between a specific microbe and a disease? Can we substitute such things as finding antibodies or DNA traces for some steps? If so, why?
C. How has microbiology contributed to the advancement in molecular biology?
Fluorescent proteins are a useful tool in the biomedical scientist's toolbox because,
a.they can be detected by flow cytometry
b.none of the above
c.they can be used to tag and track proteins
d.they can come in a variety of colors
e.all of the above
f.they can be combined with a variety of imaging methods
Chapter 15 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 15.1 - Prob. 1AYPCh. 15.1 - Provide a one-sentence description for each of...Ch. 15.2 - Identify factors that may affect the...Ch. 15.2 - Prob. 4AYPCh. 15.2 - NCLEX PREX 1. An RN is training a new staff nurse...Ch. 15.2 - NCLEX PREX 2. A clinical form used to report data...Ch. 15.3 - List at least three different tests that fall in...Ch. 15.3 - Prob. 6AYPCh. 15.3 - Discuss two major drawbacks of phenotypic testing...Ch. 15.3 - Q. What technique in this chapter do most home...
Ch. 15.3 - NCLEX PREX 3. When determining the clinical...Ch. 15.4 - Define the term serology, and explain the...Ch. 15.4 - Identify two immunological diagnostic techniques...Ch. 15.4 - Prob. 2MMCh. 15.5 - Explain why PCR is useful for infectious disease...Ch. 15.5 - Name two examples of techniques that employ...Ch. 15.5 - Explain how whole-genome sequencing can be used...Ch. 15.5 - Prob. 13AYPCh. 15.5 - Prob. 3MMCh. 15.6 - Describe the benefits of lab on a chip...Ch. 15.6 - Prob. 15AYPCh. 15 - When using pulsed-field gel electrophoresis,...Ch. 15 - Explain why it is possible to identify some...Ch. 15 - Serotyping identifies distinct members of the same...Ch. 15 - Prob. 4QCh. 15 - Name some bacterial structures that might be...Ch. 15 - You perform a lumbar puncture on a patient with...Ch. 15 - Which category of diagnosis is represented by...Ch. 15 - Write a paragraph that explains the mycobacterial...Ch. 15 - You inoculated a biochemical test strip with a...Ch. 15 - Prob. 10QCh. 15 - You perform a Kirby-Bauer disk diffusion test to...Ch. 15 - Why might culture conditions affect the results of...Ch. 15 - Which of the following techniques is most likely...Ch. 15 - Why is it more important to use selective media...Ch. 15 - What type of diagnostic method do you think would...Ch. 15 - T or F: Bacterial infection causes the expression...Ch. 15 - Prob. 17QCh. 15 - Prob. 18QCh. 15 - Prob. 19QCh. 15 - When PCR is performed by hand (not with a...Ch. 15 - What kind of a control would be important to run...
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- Discuss the significance of the following processes and what constituent is being determined: a. Borntragers’s test b. Bate-Smith and Metcalf method c. Wilstatter “cyanidin” testarrow_forwardA blood culture from a patient is taken for analysis. The potentially diseased blood is then analyzed. The lab technician discovers the presence of pathogens capable of producing alcohol. What category of diagnostic test was used to conclude this? O a. Biochemical tests O b. Microscopic analysis O c. Molecular diagnostic test such as PCR O d. None of the choicesarrow_forwardWhen determining the clinical significance of cultures, a. the number of microbes is signifi cant. b. the presence of a single colony of a true pathogen may indicate the presence of the disease if the culture comes from a site known to be sterile (i.e., cerebrospinal fl uid). c. the repeated isolation of a relatively pure culture of any microorganism can mean it is an agent of disease, although this is not always the case. d. a range of tests may be needed to identify a pathogen. e. all of the above are true.arrow_forward
- Choose the combination of answers that most accurately completes the statement.The MIC is the __________ of a drug that is required to inhibit growth of a microbe. a. largest concentration c. smallest concentration b. standard dose d. lowest dilutionarrow_forwardWhat are the ordered steps of an ELISA protocol? A. Add primary antibody->wash-> Bind sample to a surface ->Add substrate ->Add secondary antibody-enzyme conjugate ->wash B. Bind sample to support -> Add substrate -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash C. Bind sample to a surface -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash -> Add substrate D. Add secondary antibody-enzyme conjugate -> wash -> Add primary antibody -> wash -> Add substrate -> Bind sample to surfacearrow_forwardIn the Kirby-Bauer test, which of the following must be consistent? a. concentration of bacteria placed on the plate b. type of medium in the plate c. concentration of antimicrobial drug in the disk d. all of thesearrow_forward
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