Microbiology Fundamentals: A Clinical Approach
Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 7, Problem 21Q

Polymerase chain reaction (PCR) is a lab technology that requires high temperatures to make copies of DNA. The copies are made using an enzyme called a polymerase. PCR would not have been possible before thermophilic archaea were discovered. Explain why.

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As a clinical microbiologist, your job is to identify an infectious bacterial strain isolated from a patient. Phenotypically, you have discovered that the new bacterial strain can grow in the presence or absence of oxygen. By growing the strain in glucose carbohydrate tubes, you found that the organism is positive for glucose fermentation. After genome sequencing and biochemical analysis, you know that your organism is capable of making all of the enzymes necessary for glycolysis, and that it contains a TCA cycle and an electron transport chain.
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The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA: 1.  95°C for 2 minutes2.  95°C for 30 seconds3.  58.1°C for 30 seconds     4.  72°C for 45 seconds5.  Repeat steps 2-4 thirty (30) times6.  72°C for 3 minutes7.  Hold at 16°C indefinitely Which steps represent the denaturation, annealing and extension steps? a. Step 2 = Denature; Step 4 = Anneal; Step 6 = Extend b. Step 4 = Denature; Step 6 = Anneal; Step 7 = Extend c. Step 3 = Denature; Step 4 = Anneal; Step 6 = Extend d. Step 2 = Denature; Step 3 = Anneal; Step 4 = Extend

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Microbiology Fundamentals: A Clinical Approach

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Chapter 5 Microbial Metabolism; Author: Heather Davis;https://www.youtube.com/watch?v=wH_HrsfDWZw;License: Standard Youtube License