Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 9, Problem 2ITD
Summary Introduction

To review:

The organelle in which amount of CFTR protein is closest in normal and mutant cells.

Introduction:

Lungs and digestive system has mucus lining to serve various functions. In cystic fibrosis, excessive mucus is produced that results in the clogging of these systems. This condition arises due to the absence of 508th amino acid, which encodes for phenylalanine.

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You are investigating a lysosomal storage disease and you discover that newly synthesized lysosome hydrolases (proteins that should be sent to the lysosome) are secreted from the cells rather than being delivered to the lysosomes (where they should normally be located). What is the most likely explanation for this finding? (Hint:  what might be different about the proteins that would cause them to not end up in the correct location?)
A study is conducted to assess the molecular mechanisms of hormone X. A radioactively labeled form of this hormone is incubated with human cells in tissue culture for 30 minutes, and then incubated briefly with a cross-linking agent. The plasma membrane, cytosolic, and nuclear fractions are isolated from the cell lysate, and the subcellular proteins are separated from these fractions by gel electrophoresis. The only protein bound to the labeled hormone is identified in the new clear fraction. Hormone X is most likely which of the following? Epinephrine Glucagon Growth hormone Insulin Thyroxine
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