Q: Illustrate the step by step method of preparing culture media.
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Q: 1. TRUE or FALSE. An autoclave can be used to sterilize growth media?
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Q: Which of the following would be best for sterilizing a pre-sealed case of plastic medical supplies?
A: Answer - Autoclave
Q: methods of sterilization
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Q: which of the following is best to sterilize heat-liable (sensitive) solutions? a, pasteurization…
A: From above question Option d (filtration) is correct.
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Q: 1. What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame?…
A: “Since you have posted a question with multiple sub-parts, we will solve first three sub parts for…
Q: Which of the following statements is true regarding steam sterilization? a. It is a universal…
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Q: How do you sterilize the following? petri dish inoculating loop culture media test tube…
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Q: For each item on the following list, propose a reasonable method ofsterilization. You cannot use the…
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Q: 3. Give specific examples of media which are sterilized by the following method: a. Boiling or…
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Q: Solid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction…
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Q: Enlist the differences between crude media and defined media.
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Q: 9. Complete the table by transcribing the following culture media, classify as to…
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Q: describe the 3 processes that use moist heat methods specifying time and temperature for each:…
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Q: What is differential medium
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Q: 2. Illustrate preparation of a 1:32 dilution from 1-mL culture using 50-uL water blan
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Q: Name six basic nutritional requirements supplied in all culture media.
A: The culture media refers to the gel or liquid prepared for the growth of microorganisms. The culture…
Q: A fastidious organism must be grown on what type of medium? a. general-purpose medium c. synthetic…
A: The medium or the growth medium has essential nutrients for the growth of the microbes. There…
Q: 1. Can culture media be sterilized in the hot air sterilizer? Why or why not?
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Q: Agar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal…
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Q: Explain shortly the concept of differential centrifugation and how you would limit pellet…
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Q: Which of the following control agents help to be used to achieve sterility? - Virucide - Sporocide…
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Q: The following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii.…
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Q: 1. What is one advantage of utilizing the pour plate technique over the streak plate technique ?
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Q: ther than the application of heat, name other methods of sterilization. Define the methods and give…
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Q: What is the meaning of the word sterile? What would happen if culture media freshly prepared were…
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1. Briefly define culture medium.
2. What is the principle behind sterilizing media before use and before disposal?
3. Why are plated media stored in an inverted position?
Step by step
Solved in 2 steps
- 1. TRUE or FALSE. An autoclave can be used to sterilize growth media?1. What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame? 2. Why is heat a highly effective sterilizing agent?3. Which of the three methods of heat sterilization is the most appropriate and practical to use in sterilizing antibiotic solutions? (Direct flame, Dry heat, Moist heat)1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?
- 1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…1. What makes heat an effective sterilizing agent? 2. Why are the conditions required for sterilization greater for dry heat (160-180°C for 1- 2hrs) than for steam (121°C for 15min)?Illustrate the different solid tubed media formed 1. Deep/Butt solid tubed media2. Slant solid tubed media 3. Butt-slant solid tubed media
- 1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.1. What is the significance of soaking the container in bleach? 2. What is the significance of keping the container closed except when pouring the medium; partially opening the container during pour-plating in preparing culture media?1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantages of heat fixing? 3. what is the best age for your culture when performing a gram stain? Why? 4. Why is Gean staining considered a differential staining process? 5. What are some reasons a Gram positive cell might appear Gram negative?
- Can culture media be sterilized in the hot air oven? why or why not?48. Selective media is used to distinguish one microorganism type from another growing on the same media : A. False. B. True.1. Describe and give the principle of each equipment used for sterilization in microbiology. (refer to equipment drawn only)