Collected specimens are kept in seawater for 24h to void the gut before they are fixed for scanning electron microscopy (SEM) as below: • Fix in 1% OsO4 w/v in seawater for 1h • Transfer to distilled water, 6 changes over 12h • Store in 70% (v/v) ethanol There are 150 specimen that need to be fixed for SEM. You'll be using Petri dishes (diam. 15cm) which need to be filled 1 cm high to cover the animals. a) How much fixative do you need to prepare in litres (to one decimal place)? Hint: need volume of cylinder - r2 π h b) How much OsO4 do you need to weigh out to make your fixative? (in g)
Collected specimens are kept in seawater for 24h to void the gut before they are fixed for scanning electron microscopy (SEM) as below: • Fix in 1% OsO4 w/v in seawater for 1h • Transfer to distilled water, 6 changes over 12h • Store in 70% (v/v) ethanol There are 150 specimen that need to be fixed for SEM. You'll be using Petri dishes (diam. 15cm) which need to be filled 1 cm high to cover the animals. a) How much fixative do you need to prepare in litres (to one decimal place)? Hint: need volume of cylinder - r2 π h b) How much OsO4 do you need to weigh out to make your fixative? (in g)
Microbiology for Surgical Technologists (MindTap Course List)
2nd Edition
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Margaret Rodriguez, Paul Price
Chapter3: The Microbiology Laboratory
Section: Chapter Questions
Problem 4TBP
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Collected specimens are kept in seawater for 24h to void the gut before they are fixed for scanning electron microscopy (SEM) as below:
• Fix in 1% OsO4 w/v in seawater for 1h
• Transfer to distilled water, 6 changes over 12h
• Store in 70% (v/v) ethanol
There are 150 specimen that need to be fixed for SEM. You'll be using Petri dishes (diam. 15cm) which need to be filled 1 cm high to cover the animals.
a) How much fixative do you need to prepare in litres (to one decimal place)? Hint: need volume of cylinder - r2 π h
b) How much OsO4 do you need to weigh out to make your fixative? (in g)
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