(a)
Interpretation:
The primers frequently chosen that have high number of cytosines and guanines in their sequences; the reason has to be predicted.
Concept introduction: Cytosine is a
Primers are short strand(
(b)
Interpretation:
The primer sequence of CCCCCCCCCCGGGGGGGGGG not is a good choice; the reason has to be predicted.
Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.
Primers are short strand(
(c)
Interpretation:
Vast excess of primer used for the amount of DNA has to be amplified.
Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.
Primers are short strand(
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Chemistry & Chemical Reactivity
- Are the following compounds correct? These may OR may not be correct. Please look them over and correct them if needed. Please list what was done incorrectly if applicable. b. Is the following nucleotide found in DNA? If not, please explain why. a. Is the following tRNA correct? NH2 VALINE <5' N. 3'- 0=P-0-CH2 ОН ОН GUA a. b. DNAA=T G=Carrow_forward1. Draw the two Watson-Crick Bases Pairs and show how G- C and A-T recognize each other by hydrogen bonding. 2. Draw the full structure of two nucleotides - one from DNA and one from RNA. Point out the key difference between the two. 3. Write out an example of a condensation reaction forming a phosphate-sugar bond. 4. Write out an example of a condensation reaction between a nucleic acid base and a sugar. 5. State the "Central Dogma" of biochemistry.arrow_forwardThe basic principle that underlies northern and Southern blots is . A. laser excitation B. hybridization via base pairing C. fluorescently labeled antibodies D. dideoxynucleotide chain terminationarrow_forward
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- 16. Describe the basic principles behind the functioning of an amino acid analyzer. What is it? W- is it used for? How does it work (start to finish)? 17. In peptide, protein chemistry, what does the term 'sequence' mean? 18. About how many amino acid residues can be in a peptide in order to sequence it by Edman degradation?arrow_forwardWhat are annulenes ?arrow_forwardWhich of the following amino acid has an aromatic group? * (2 Points) valine O lysine threonine O tyrosinearrow_forward
- 3. Tm is the temperature at which all of a certain DNA issingle-stranded. a. true b.false 4. Type II restriction endonuclease is useful for molecular cloning. a. true b.falsearrow_forwardProtein sequence is always read (blank) terminal to (blank) terminal. And DNA sequence is always read (blank) prime to ( blank) prime. I need help filling in the blanks please? ? Thanksarrow_forwardWhat is qrxn?arrow_forward
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