The change in Gibbs free energy for the given reaction needs to be determined. The number of ATP molecules formed under standard conditions needs to be determined. Concept introduction: The relation between the change in Gibbs free energy and standard redox potential is as follows: Δ G = − n F Δ E ° Here, n is number of electrons transferred, F is Faraday’s number and Δ E ° is standard redox potential.

Question

A mutant version of DADH can use NADP+ as a cofactor for isopropanol oxidation. Velocity data was collected from reactions at a series of NADP+ concentrations. The following trendline was obtained for a Lineweaver-Burk plot of the data: y = 0.00007x + 0.0014 Note that the NADP+ substrate concentrations are in mM and the reaction velocity was measured in nmol/min. Calculate the Km and Vmax for DADH with this substrate. Show your work.

Answer 1

Question

Chapter 27, Problem 4P

Interpretation Introduction

Interpretation:

The change in Gibbs free energy for the given reaction needs to be determined. The number of ATP molecules formed under standard conditions needs to be determined.

Concept introduction:

The relation between the change in Gibbs free energy and standard redox potential is as follows:

ΔG=−nFΔE°

Here, n is number of electrons transferred, F is Faraday’s number and ΔE° is standard redox potential.

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