Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 9, Problem 3TY
Summary Introduction
Introduction:
The DNA double helix consists of sugar-phosphate backbones, which are alternating units of deoxyribose and phosphate joined with covalent bonds. The two chains of DNA are arranged parallel to each other but oriented in the opposite direction. The two chains are joined by hydrogen bonds.
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Which of the following are possible base compositions for double-
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Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical?
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Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
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Chapter 9 Solutions
Principles of Biology
Ch. 9.1 - Look ahead to Figure 17.10. How does bacterial...Ch. 9.1 - Prob. 1TYKCh. 9.2 - Prob. 1CCCh. 9.2 - Prob. 2CCCh. 9.2 - Prob. 3CCCh. 9.2 - A nucleotide composed of deoxyribose, phosphate,...Ch. 9.2 - Prob. 2TYKCh. 9.2 - Prob. 3TYKCh. 9.3 - Prob. 1TYKCh. 9.3 - To determine the structure of DNA, Watson and...
Ch. 9.4 - Prob. 1CCCh. 9.4 - Prob. 1TYKCh. 9.4 - Prob. 2TYKCh. 9.5 - Prob. 1CCCh. 9.5 - Prob. 1TYKCh. 9.5 - Prob. 2TYKCh. 9.5 - Prob. 3TYKCh. 9.6 - Prob. 1CCCh. 9.6 - A nucleosome is composed of 146 bp or 147 bp of...Ch. 9.6 - After they have replicated and become compacted in...Ch. 9.6 - Prob. 1BCCh. 9.6 - Which of the following is the correct order for...Ch. 9 - What is/are the main component(s) of chromosomes?...Ch. 9 - Prob. 2TYCh. 9 - Prob. 3TYCh. 9 - Prob. 4TYCh. 9 - Of the following statements, which is correct when...Ch. 9 - Prob. 6TYCh. 9 - Prob. 7TYCh. 9 - Prob. 8TYCh. 9 - Prob. 9TYCh. 9 - The conversion of euchromatin into heterochromatin...Ch. 9 - What are the four criteria that the genetic...Ch. 9 - What are the key features of DNA that allow it to...Ch. 9 - PRINCIPLES A principle of bioloy is that structure...Ch. 9 - Prob. 1CBQCh. 9 - Prob. 2CBQ
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- You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A26o) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table belowarrow_forwardWhy evenly spaced sequence in chromatogram is an indication of a good DNA chromatogram?arrow_forwardYou extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (Az60) of the solution in the cuvette is 0.2 (Remember: an Az60 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out cach of the boxes in the Table below What to Calculate | Calculation / Result DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40% In your extraction of DNA from wheat germ, would you expect that nuclear DNA is the only source…arrow_forward
- That's the result of Gel electrophoresis of genomic DNA ( Of genomic DNA extraction experiment), please discuss the results and label and name the image to illustrate the answer? - Marker band sizes in gel: From top (well side) to bottom the bands have the following size in base-pair/bp- 6751,3652,2827,1568,1118,825,630arrow_forwardYou extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below What to Calculate Calculation / Result DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40%arrow_forwardAll of the following are examples of materials that are bound by your purifying medium during the DNA extraction process, except: O endoplasmic reticulum extraneous DNA O Calcium (Ca2+) O Iron (Fe2+) O Magnesium (Mg2+) O lipid membranesarrow_forward
- You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below What to Calculate Calculation / Result Marks DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40%arrow_forwardBelow is an EMSA showing four different reactions, A-D. In each tube there is some combination of labelled DNA probe, Protein X (the protein you are studying), and an antibody for Protein X. Identify which combination of components are found in each of the four reactions and explain how you determined that based on the molecular interactions being studied and your knowledge of gel electrophoresis. It is possible that multiple lanes have the same component(s). A B C D EMSAarrow_forwardBoth protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.arrow_forward
- A plot showing the % of denaturation as a function of temperature for a melting point of a DNA sample under 0.12 M NaCl gives an equation of a line of Y= 0.0074 X - 0.044. (where Y is the % of denaturation and X is the temperature in °C) Question: Calculate the melting point of this DNA in °C.arrow_forwardYou perform an experiment to determine the complete melting profile of a relatively long DNA duplex with 80% G:C base pairs. Sketch a graph of Absorbance at 260 nm (Y-axis) vs. Temperature (X-axis). Label the location of the Tm and describe what is happening to the DNA structure in each segment of the graph (lower Temps, highest Temps, at Tm).arrow_forwardYou extract DNA from 200, You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260) of the solution in the cuvette is 0.2 (Remember: an A 260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below What to Calculate Calculation / Result Marks (x / 100) 12/100 DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette 12/100 12/100 Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ 24/100 assuming an extraction efficiency of 40%arrow_forward
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